Application of Gel Permeation HPLC for Lipoprotein Profiling in Dogs
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概要
- 論文の詳細を見る
A high performance liquid chromatography system with a gel permeation column (GP-HPLC) and an on-line dual enzymatic system was applied to lipoprotein analysis in dogs. A high density lipoprotein (HDL) fraction obtained by conventional ultracentrifugation gave a single peak at around 28–29 min. Similarly, a low density lipoprotein (LDL) fraction gave single peak at around 24–25 min. The lipoprotein profiles of healthy dogs were contained large HDL peaks and small LDL peaks, and VLDL and CM were only marginally detected. In diabetic dogs, concentrations of VLDL-triglyceride and VLDL-total-cholesterol were elevated significantly. The lipoprotein profile analysis by GP-HPLC method would be useful in explication of abnormality of lipid metabolism in dogs.
- 社団法人 日本獣医学会の論文
著者
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SAKO Toshinori
Department of Veterinary Nursing, School of Veterinary Nursing and Technology, Nippon Veterinary and
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MORI Akihiro
Department of Gastroenterology, Inuyama Chuo Hospital
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Mori Akihiro
Department Of Electric Engineering Faculty Of Engineering Kyoto University
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Sako T
Nippon Veterinary And Animal Sci. Univ. Tokyo Jpn
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Sako Toshinori
Veterinary Internal Medicine Nippon Veterinary And Animal Science University
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MIZUTANI Hisashi
Veterinary Internal Medicine, Nippon Veterinary and Life Science University
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ARAI Noriaki
Spectrum Lab. Japan K.K.
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KURIYAMA Koji
Skylight Biotech K.K.
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YOSHIMURA Itaru
Fuji Animal Farm, Nippon Veterinary and Life Science University
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IWASE Kenji
Veterinary Internal Medicine, Nippon Veterinary and Life Science University
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HIROSE Hisashi
Veterinary Internal Medicine, Nippon Veterinary and Life Science University
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MORI Akihiro
Veterinary Nursing, Nippon Veterinary and Life Science University
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