Chromosomal Analysis of First-Cleavage Mouse Eggs Fertilized in Caffeine-Containing Medium
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概要
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Eggs derived from superovulated F<SUB>1</SUB>(BALB/c × C57BL/6) mice were fertilized in a modified Krebs-Ringer bicarbonate medium (TYH) containing 2-mM caffeine. Spermatozoa obtained from the cauda epididymis of ICR male mice were preincubated in the caffeine-containing for 2 h before the mixing of gametes. Gametes preincubated and inseminated in the caffeine-free medium served as controls. Chromosome analysis at the first cleavage showed a significantly (P<0.05) higher incidence of triploidy in eggs fertilized in the caffeine-containing medium (22.9%) than in the control (15.8%). The incidence of aneuploidy and the sex ratio were not significantly different between the two groups of eggs. Most of the triploid eggs observed were considered to be dispermic in origin.
著者
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Fukui Emiko
Department of Animal Science, Faculty of Agriculture, Utsunomiya University
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Yoshizawa Midori
United Graduate School Of Agriculture Tokyo University Of Agriculture And Technology
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OKAMOTO Akira
Laboratory of Clinical Chemistry, National Cerebral and Cardiovascular Center
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Yoshizawa Midori
Laboratory Of Animal Breeding And Reproduction Division Of Animal Science Faculty Of Agriculture Uts
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NAKAMOTO Satoshi
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
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MURAMATSU Takashi
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
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OKAMOTO Akira
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University
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FUKUI Emiko
Department of Legal Medicine and Human Genetics, Jichi Medical School
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