Analysis of the tyrosinase gene of the Japanese pond frog, Rana nigromaculata: Cloning and nucleotide sequence of the genomic DNA containing the tyrosinase gene and its flanking regions.
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概要
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Three genomic DNA fragments containing the tyrosinase-encoding gene (<i>TYR</i>) of the Japanese pond frog, <i>Rana nigromaculata</i>, were cloned. The first, clone I, was isolated from a genomic library of sperm DNA using the mouse <i>TYR</i> cDNA as the probe and contained a DNA segment similar to exon 4 of the mouse <i>TYR</i> gene. Subsequently, the <i>TYR</i> cDNA was isolated by screening a frog embryo cDNA library using clone I as the probe. Two clones that contain genomic DNA of the <i>TYR</i> gene were isolated also from a blood cell DNA library using the frog <i>TYR</i> cDNA as the probe. Comparison of the nucleotide (nt) sequences of the genomic clone II DNA and the cDNA revealed that clone II contained a 3,140-bp DNA fragment consisting of the 5'-flanking region, the first exon, and a part of the first intron. The region upstream of the coding region contained the characteristic sequences for regulatory elements, including TATA- and CAAT-motifs, and also a pigment cell-specific promoter element, which is shared by the promoter regions of the vertebrate <i>TYR</i> genes. A 764-bp segment containing an upstream 748-bp non-coding region and 16-bp coding region was functional for expression of the promoter-less <i>cat </i>gene on a plasmid in the transiently transformed albino frog melanophore. The genomic clone III contained the 3'-untranslated region of the mRNA and its 3' flanking region. Thus, the cDNA plus genomic DNA fragments isolated here cover the entire <i>TYR</i> gene and its flanking regions.<br>
- 日本遺伝学会の論文
著者
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Nishioka Midori
Laboratory For Amphibian Biology Faculty Of Science Hiroshima University
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Miura Ikuo
Laboratory For Amphibian Biology Faculty Of Science Hiroshima University
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Okumoto Hitoshi
Laboratory For Amphibian Biology Faculty Of Science Hiroshima University
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MAKINO Kozo
Research Institute for Microbial Diseases, Osaka University
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NAKATA Atsuo
Faculty of Pharmacology and Pharmaceutical Sciences, Fukuyama University
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