ミセル動電クロマトグラフィーの生薬分析への応用(第1報) : 漢方製剤中のglycyrrhizinの定量

概要

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High-performance capillary electrophoresis (HPCE) using a micellar solution of ionic surfactant, called micellar electrokinetic chromatography (MEKC), was applied to the analysis of glycyrrhizin in Glycyrrhizae Radix and commercial oriental pharmaceutical preparations containing Glycyrrhizae Radix. A laboratory-made instrument was used. A high voltage (ca. 180 V/cm) was applied to a 700 mm × 0.05 mm I.D. fused silica capillary tube filled with a borate-phosphate buffer solution (pH 9) containing 100 him of sodium dodecyl sulfate. A sample was introduced into the tube by siphoning. The on-column detection was performed at 254 nm by using n-propyl p-hydroxybenzoate as an internal standard. Both of the calibration line obtained from the peak height ratios and that from the peak area ratios gave a good linearity over the concentration range, 50-1,000 μg/ml. The coefficients of variation for the determination of glycyrrhizin contents ir> six commercial preparations by this method were 2.7-7.0%.
日本生薬学会の論文
1991-09-20