黄連末のT.L.C.による純度試験法

概要

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A quick reliable method is needed for the detection and identification of Coptidis Rhizoma Pulveratum. Thin layer Chromatography is applied to this purpose. Macerate 20 g. of the dried drugs, finely powdered, with 700 ml of ethyl acetate following by the determination of alcohol-soluble extractive by J. S. P. 1000 ml filtrate is evaporated to 10 ml and transfer to 4 g. of the adsorbant (Florisil, 60-100 mesh for chromatography, activated at 130℃, 3hr.) in a small flask. Pour the content of the flask on a wetted filter with ethyl acetate and wash the residue with about 50 ml of ethyl acetate. Evaporate the total filtrate to dryness in vacuum at 45-50℃. Dissolve the residue in 2 ml of ethyl acetate. Arrange for T. L. C. using silica gel G (Merk), through a freshly prepared solution consising of 30 volumes of toluene, 10 volumes of ethyl formate, 10 volumes of formic acid and 2.5 volumes of pyridine and spot 10 pl of the preparation on the plate. Compare the test preparation with the standard preparation after visualizing with c-H_2SO_4. By this method, a small portion of powdered crude drugs, for instance Phellodendri Cortex Pulveratum, Rhei Japonicae Rhizoma and Curcumae Rhizoma, in Coptidis Rhizoma Pulveratum is easily detectable.
日本生薬学会の論文
1973-06-20