Determination of Sinomenine in Sinomeni Caulis et Rhizoma and in Chinese Medicinal Prescriptions by Micellar Electrokinetic Chromatography

概要

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Sinomenine contents in Sinomeni Caulis et Rhizoma and in Chinese medicinal prescriptions was determined by utilizing micellar electrokinetic chromatography. An AccuSep capillary column (75μm i.d.×60crn) was employed, 5mM sodium dihydrogenphosphate-20mM sodium tetraborate (pH9.1) containing 50 mM sodium dodecyl sulfate was used as the electrophoretic buffer. The migration was performed at 20 kV and sinomenine was detected by UV absorption at 254 nm (on column). Sample solution was injected through the hydrostatic method (10s). The sinomenine contents in commercially available Sinomeni Caulis et Rhizoma samples (0.13-0.39%), and those in decoctions of Boi-bukuryo-to (4.16±0.33mg), Boi-ogi-to (6.57±0.28mg) and Moku-boi-to (5.53±0.20mg) could be estimated rapidly and briefly. However, the sinomenine content in a decoction of Sokei-kakketsu-to consisting of many crude drugs could not be accurately estimated by this method.
日本生薬学会の論文
1997-12-20