Effect of Bone Morphogenetic Protein-2 (BMP-2) or Troglitazone, as an Inducer of Osteogenic Cells or Adipocytes, on Differentiation of a Bone Marrow Mesenchymal Progenitor Cell Line Established from Temperature-Sensitive (ts) Simian Virus (SV) 40 T-Antige

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概要

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• TBR31-2 is one of the bone marrow stromal cell lines. Differentiation toward osteogenic cells and calcification was observed when TBR31-2 cells were cultured for 4 weeks. Bone morphogenetic protein-2 (BMP-2) stimulated alkaline phosphatase (ALP) activity in a dose- and time-dependent manner. On the other hand, troglitazone increased oil droplet accumulation in a dose-dependent manner. In the presence of BMP-2, an increase of expression in osteogenic cell differentiation marker genes and a decrease of expression in adipocyte differentiation marker genes were observed with the exception of the induced expression of peroxisome proliferator-activated receptor γ (PPARγ), however, troglitazone, a ligand of PPARγ treatment exhibited the opposite tendency. Interestingly, treatment with both BMP-2 and troglitazone resulted in a decrease of ALP activity and an increase of oil droplet accumulation. Reverse tanscription-polymerase chain reaction (RT-PCR) analysis also indicated that osteogenic differentiation markers decreased and that adipocyte differentiation markers increased. Thus, when the cells were cultured with BMP-2, osteogenic differentiation was enhanced while the expression of PPARγ was maintained, and the addition of troglitazone caused a significant number of differentiated cells into adipocytes. These findings indicate that BMP-2 enhanced osteogenic differentiation and the expression of adipogenic transcription factor (PPARγ) followed by osteogenic differentiation without activation of PPARγ by its ligand.

• 公益社団法人日本薬学会の論文
• 2009-01-01

著者

• 戸苅 彰史

  愛知学院大学歯学部薬理学講座

• 新井 通次

  愛知学院大 歯 薬理

• 戸苅 彰史

  愛院大・歯・薬理

• YANAI Nobuaki

  Department of Cell Biology, Institute of Development, Aging and Cancer

• Yanai Nobuaki

  武庫川女子大学 薬学部生化学i

• Yanai Nobuaki

  Department Of Food And Nutritional Science Faculty Of Liberal Arts Miyagi Gakuin Women's Colleg

• Yanai Nobuaki

  Department Of Biological Science Tohoku University

• Togari Akifumi

  Department of Pharmacology, School of Dentistry, Aichi-Gakuin University

• Togari Akifumi

  武庫川女子大学 薬学部生化学i

• Togari Akifumi

  Department Of Pharmacology School Of Dentistry Aichi-gakuin University

• Togari Akifumi

  Departments Of Pharmacology School Of Dentistry Aichi-gakuin University

• Togari Akifumi

  Department Of Chemical Pharmacology Faculty Of Pharmaceutical Sciences Nagoya City University

• Togari Akifumi

  Departments Of Anatomy Pharmacology School Of Dentistry Aichi-gakuin University

• ARAI Michitsugu

  Department of Pharmacology, School of Dentistry, Aichi-Gakuin University

• NISHII Naomi

  First Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's U

• NAKABAYASHI Toshikatsu

  First Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's U

• Arai Michitsugu

  Department Of Pharmacology School Of Dentistry

• Nishii Naomi

  First Department Of Biochemistry School Of Pharmaceutical Sciences Mukogawa Women's University

• 戸苅 彰史

  名古屋市立大学薬学部薬品作用学教室

• Nakabayashi Toshikatsu

  First Department Of Biochemistry School Of Pharmaceutical Sciences Mukogawa Women's University

• Nishi Naomi

  First Department of Biochemistry, School of Pharmaceutical Sciences, Mukogawa Women's University

• Yanai Nobuaki

  Department of Food and Nutritional Science, Faculty of Liberal Arts, Miyagi Gakuin Women's College

• Nejime Namie

  Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University

• NAKABAYASHI Toshikatsu

  First Department of Biochemistry, School of Pharmaceutical Sciences, Mukogawa Women's University

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