A Novel Stromal Cell-Dependent B Lymphoid Stem-Like Cell Line That Induces Immunoglobulin Gene Rearrangement.
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概要
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A stroma-dependent B lymphoid cell line (B31-1) has been established by coculturing sorted stem cells on a novel bone marrow stromal cell line (TBR31-1). B31-1 cells express B220, but do not express other B lymphoid differentiation markers including CD43, heat stable antigen (HSA), or surface immunoglobulin (Ig) M (sIgM), and their Ig heavy chain (IgH) gene loci are germ-line in configuration. The addition of interleukin (IL)-7 or coculture with another stromal cell line, ST2, induces D-J rearrangement of the IgH gene and B lymphocyte differentiation markers. B31-1 cells restore an in vivo repopulation activity to lethally irradiated mice, and the repopulated cells differentiate to HSA+ pre-B cells. Continuous coculture results in two distinct populations, B220- c-Kit- cells and B220+ c-Kit+ cells; B220- c-Kit+ cells are self-renewed and differentiate to B220+ c-Kit+ cells, while B220+ c-Kit+ cells produce only B220+ c-Kit+ cells. Both B220- and B220+ cells similarly express the IgH germ-line transcript (Iμ), mRNAs for recombinase (TdT, Rag-1, and Rag-2), and lymphoid-specific transcription factors (Pax-5, EBF, E12/E47, Oct-2, and Ikaros), but the DNA binding activity of Pax-5, EBF, Oct-2, and E2A are low in B220- cells and while high in B220+ cells. These results suggest the existence of at least two active states in the IgH locus before the induction of IgH gene rearrangement during B lymphopoietic development.
- 社団法人 日本生化学会の論文
著者
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Yanai Nobuaki
Department Of Biological Science Tohoku University
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Obinata Masuo
Department Od Cell Biology The Research Institute For Tuberculosis And Cancer Tohoku University
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Okuyama Ryuhei
Department Of Cell Biology Institute Of Development Aging And Cancer
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Matsuda Ken-ichi
Department Of Anatomy And Neurobiology Graduate School Of Medical Science Kyoto Prefectural Universi
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Koguma Masahito
Department Of Cell Biology Institute Of Development Aging And Cancer
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Nakazawa Tomoko
Department Of Anatomy And Developmental Biology School Of Medicine Tokyo Women's Medical Univer
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Terasaki Tetsuya
Faculty Of Pharmaceutical Sciences Kanazawa University
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Nakauchi Hiromitsu
Department Of Immunology University Of Tsukuda
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Matsuzaki Yumi
Department Of Immunology University Of Tsukuda
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