P-44 組織培養を利用した機能性イソプレノイドの合成(ポスター発表の部)

概要

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In order to obtain biologically functional isoprenoid compounds by use of a convenient system, we examined biocatalytic syntheses by employing some isoprenoid diphosphates, which are used as substrates for the enzymatic reactions in the main route of the early stage of isoprenoid biosynthesis as the substrates of the biocatalytic synthesis using some plant cultures developed from some vegetables such as pumpkin or carrot. After administration of the substrates [12.5(allylic substrate) or 25 (homoallylic substrate) μmoles/mL culture medium], the cultures were incubated at 24℃ for 20 days on a rotary shaker (70rpm) in the dark. The biocatalytic reaction of geranyl diphosphate (GPP) with isopentenyl diphosphate (IPP) by use of tissue culture developed from pumpkin shoot gave 3 kinds of prenylalcohols, farnesol (FOH: yield: 0.13%), geranylgeraniol (GGOH: 7.83%), and geranylfarnesol (GFOH: 0.04%), and 2 kinds of prenylcarboxylic acids, farnesoic acid (FA: 0.02%) and geranylgeranoic acid (GGA: 0.04%). The biocatalytic reaction of dimethylallyl diphosphate (DMAPP) with IPP using the similar cell culture as above afforded GOH (yield: 7.24%), FOH (1.17%), and GGOH (1.50%), respectively.
天然有機化合物討論会の論文
2006-09-15