P-2 組織培養によるイソプレノイド類の合成研究(その2)(ポスター発表の部)

概要

論文の詳細を見る
In the biosynthesis of isoprenoid compounds construction of the fundamental prenyl chain backbone of short chain prenyl compounds is catalyzed by several kinds of prenyltransferases or prenyl chain elongating enzymes, which catalyze the sequential condensation of isopentenyl diphosphate (IPP) with an allylic prenyl diphosphate such as dimethylallyl diphosphate (DMAPP, C_5) or geranyl diphosphate (GPP,C_<10>). To develop convenient biotransformation systems for the syntheses of such biologically active isoprenoid compounds with short prenyl chain backbone, we fed IPP and the allylic diphosphates (DMAPP or GPP) directly to tissue-cultured cells of pumpkin, Cucurbita maxima. After long term incubations followed by alkaline phosphatase treatment, we detected some of the biocatalytic reaction products such as geraniol (GOH), farnesol (FOH), geranylgeraniol (GGOH), and geranylfarnesol (GFOH) in the cultured mediums fed with DMAPP and IPP, or with GPP and IPP. Furthermore, from the cultured medium fed with GOH and isopentenol (IOH), we obtained GGOH along with small amounts of FOH and GFOH. These results suggest that diphosphorylation of these short chain prenyl alcohols occurred in the cultured cells, followed by the prenyl chain elongation by the action of some prenyltransferases.
天然有機化合物討論会の論文
2007-08-24