96 ファルネシル二リン酸合成酵素の人工基質(ポスター発表の部)

概要

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The substrate specificity of farnesyl diphosphate synthase of pig liver was studied with artificial allylic diphosphate having hetero atom, oxygen or sulfur, in their side chain. 1. The DMAPP analogues 1, 2, 3 and 4 were found to be substrate for this enzyme (Fig. 1). 2. Three isomers of DMAPP analogues 5, 6 and 7 were prepared and examined. These analogues were also found to be substrate for this enzyme. In the presence of Mg^<2+>, the 5 was condensed with one molecule of IPP to form geranyl-PP type product. However, the same analogue gave farnesyl-PP type product in the presence of Zn^<2+> (Fig.2, 3). 3. The GPP analogues having hetero atoms were also shown to be substrate for this enzyme (Fig.4). 4. The DMAPP analogues having an unprotected hydroxyl group, 18, was also accepted as substrate to produce farnesyl-PP type product. The analogue 19, modified 18 with MOMO group, was condensed with one molecule of IPP to form geranyl-PP type product (Fig.5). These results suggest that this enzyme can have such a very wide specificity for allylic diphosphate that it may be applied to bioorganic synthesis.
天然有機化合物討論会の論文
1993-09-10