45 アジサイが多彩な色を発現する機構(口頭発表の部)

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Hydrangea flower color is varied from red, through purple, to blue. However, all colors are caused from the same pigment, delphinidin 3-glucoside (1). By extraction from the color sepals, Hydrangea macrophylla, 5-O-caffeoylquinic acid (2), chlorogenic acid (3-O-caffeoylquinic acid, 4) and trace amounts of 5-O-p-coumaroylquinic acid (3), accompanied with 1, were isolated. By using of their co-pigments in the presence of Al^<3+> the flower color reproduction in vitro was performed at varieties of pHs. At pH 4, the blue color was realized using of 2 as a co-pigment, and at pH 3 purple. However, at pH 3, 1 with 4 or without co-pigment in the presence of Al^<3+> showed red color, at pH 4 purple pigment was precipitated. Lacking of Al^<3+> at the above pH range it was quickly decolorized. By reproduction of the blue color using various synthetic analogues as a co-pigment the molecular structure of blue color complex could be proposed. The color variation of hydrangea caused by nonstoichiometric supra molecule and the blue pigment may be an Al^<3+> complex associated with copigments, 5-O-caffeoylquinic acid (2) and 5-O-p-coumaroylquinic acid (3). From the blue and red sepals protoplasts were prepared. The components of blue protoplasts (ca 100 to 200 cells) were analyzed with use of micro-HPLC and ICP-MS. The concentration of 1 was 5×10^<-3> M and ratio of 1, 2, 3, 4 and Al^<3+> was deduced to be ca 1:2:0.1:5:1. By concerning with red sepals 1 was 5×10^<-3> M and ratio of 1, 2, 3, 4 and Al^<3+> was ca 1:0.5:0.3:10:0.1. In vitro reconstruction using those condition described above gave the same colored blue or red solution as both protoplast measured by microscopic absorption spectrometer. In conclusion, the blue color might be developed with a complex from 1, 2, 3 and Al^<3+> at pH 4 and the red color might be due to 1, 4 and Al^<3+>. The color-change and variation of hydrangea may be caused by loose Al^<3+> complexation of those components responding to slight vacuolar pH change.
天然有機化合物討論会の論文
1999-09-01

45 アジサイが多彩な色を発現する機構(口頭発表の部)

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