P-83 チューリップ花弁の青色発色と鉄輸送機構の解明(ポスター発表の部)

概要

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We have been studying mechanisms underlying a blue coloration of tulip, Tulipa gesneriana cv. Murasakizuisho that has blue color in the cells at the bottom of the inner perianth and purple color in the cells of the upper part. We purified the petal pigment and co-pigments and the structure was determined to be delphinidin 3-O-rutinoside (1), manghaslin (2), rutin (3), and mauritianin (4). From blue and purple part of the petals we prepared each colored protoplast and analyzed vacuolar pH, content of anthocyanin and co-pigments, and metal elements of them. The vacuolar pH values of the purple and blue protoplasts were 5.5 and 5.6, respectively; without any significant difference. The content of pigment and co-pigment was also the same. However, the Fe content in the blue protoplast was 25 times higher than that in the purple protoplasts. Furthermore, we could reproduce the blue and purple solution by mixing 1-4 with/without Fe^<3+>, respectively. We clarified that Fe^<3+> is essential for blue color development in the tulip (Shoji et al., PCP, 48, 2007). These results strongly suggested of vacuolar iron transporter in blue color development of tulip petals and now the study is in progress.
2008-09-01