76 四国産海綿の細胞毒性新規マクロラクタム化合物の構造(ポスター発表の部)

概要

論文の詳細を見る
During the course of our screening program for bioactive metabolites from Japanese marine invertebrates, we isolated a cytotoxic macrocyclic lactam from a sponge. The isolation and structure elucidation have been attempted. The MeOH extract was concentrated in vacuo, and diluted with water. The solution was extracted with ether; insolube middle layer was obtained as a wax during extraction. The wax was purified by reverse-phase chromatographies to yield a macrocyclic lactam. The lactam had a molecular formula of C_<27>H_<34>O_5N_2 which was established by the HRFABMS, and NMR spectra. Its gross structure was determined mainly by interpretation of 2D NMR spectra to be a macrocyclic lactam including a tetramic acid and tri-substituted bicyclo[3, 3, 0]octene skelton. The relative stereochemistry of tri-substituted bicyclo[3, 3, 0]octene portion was deduced by the NOESY data. This lactam was cytotoxic against B16 melanoma cells with IC_<50> of 0.8 μg/ml.
天然有機化合物討論会の論文
1992-09-10