Effects of Eleven Flavonoids from the Osteoprotective Fraction of Drynaria fortunei (KUNZE) J. SM. on Osteoblastic Proliferation Using an Osteoblast-Like Cell Line

スポンサーリンク

概要

• 論文の詳細を見る

• Drynaria fortunei (KUNZE) J. SM. (DFE) is one of the most frequently used traditional Chinese medicines prescribed for the treatment of osteoporosis in China. The present study was designed to investigate the osteoprotective constituents from Drynaria fortunei. The 60% ethanol extract of the rhizome of D. fortunei (DFE) was chromatographed on a D-101 macroporous resin column (φ25×150cm) ; three fractions (DFA eluted with water, DFB eluted with 30% and 50% ethanol, and DFC eluted with 95% ethanol) were obtained and their osteoprotective activity as examined both in vivo and in vitro. DFB showed significant activity on both the proliferation of UMR106 cells and promoting bone mineral density (BMD) in ovariectomized (OVX) mice. A bioactivity-guided method led to the isolation of 11 flavonoids from this fraction (DFB) with antiosteoporotic activity, including two new compounds, kaempferol 3-O-β-D-glucopyranoside-7-O-α-L-arabinofuranoside (1) and (R)-5,7,3',5'-tetrahydroxy-flavanone 7-O-neohesperidoside (2), along with nine known ones: three flavanones (3-5), one flavone (7), one flavonol (6), two chromones (8, 10), maltol glucoside (9), and (-)-epicatechin (11). Compounds 4-11 are reported for the first time from this genus. We investigated the proliferative effects of the 11 compounds in the UMR106 osteoblastic cell line in vitro. All compounds exhibited the proliferative activity in the UMR106 cells at most of the concentrations tested. Most compounds are reported for the first time from the Drynaria genus and this was the first study of their proliferative activity in osteoblast-like cells. The main peaks in the HPLC fingerprint of the active fraction (DFB) were also identified.

• 2008-01-01

著者

• WANG Xin-Luan

  School of Chinese Materia Medica, Shenyang Pharmaceutical University

• WANG Nai-Li

  School of Chinese Materia Medica, Shenyang Pharmaceutical University

• ZHANG Yan

  State Key Laboratory of Chinese Medicine and Molecular Pharmacology

• GAO Hao

  Key Lab for New Drugs Research of Traditional Chinese Medicine, Shenzhen Research Institute of Tsing

• PANG Wai-Yin

  Central Laboratory, Institute of Molecular Technology for Drug Discovery and Synthesis, Department o

• WONG Man-Sau

  Central Laboratory, Institute of Molecular Technology for Drug Discovery and Synthesis, Department o

• ZHANG Ge

  Musculoskeletal Research Laboratory, Department of Orthopedics and Traumatology, Chinese University

• QIN Ling

  Musculoskeletal Research Laboratory, Department of Orthopedics and Traumatology, Chinese University

• YAO Xin-Sheng

  School of Chinese Materia Medica, Shenyang Pharmaceutical University

• Yao Xin

  Medical College Jinan University

• Qin Ling

  Musculoskeletal Research Laboratory Department Of Orthopaedics & Traumatology The Chinese Univer

• Qin Ling

  Musculoskeletal Research Laboratory Department Of Orthopedics And Traumatology Chinese University Of

• Zhang Ge

  Musculoskeletal Research Laboratory Department Of Orthopedics And Traumatology Chinese University Of

• Zhang Yan

  State Key Lab. Of Chinese Medicine And Molecular Pharmacology

• Yao Xin-sheng

  Inst. Of Traditional Chinese Medicine And Natural Products Jinan Univ.

• Yao Xin-sheng

  School Of Traditional Chinese Materia Medicine Shengyang Pharmaceutical Univ.

• Wang Nai-li

  School Of Chinese Materia Medica Shenyang Pharmaceutical University:key Lab For New Drugs Research O

• Wong Man-sau

  Central Lab. Inst. Of Molecular Technol. For Drug Discovery And Synthesis Dep. Of Applied Biology An

• Wong Man-sau

  Central Laboratory Institute Of Molecular Technology For Drug Discovery And Synthesis Department Of

• Pang Wai-yin

  Central Laboratory Institute Of Molecular Technology For Drug Discovery And Synthesis Department Of

• Wang Xin-luan

  School Of Chinese Materia Medica Shenyang Pharmaceutical University

• Gao Hao

  Key Lab For New Drugs Research Of Traditional Chinese Medicine Shenzhen Research Institute Of Tsingh

• Yao Xin-sheng

  School Of Chinese Materia Medica Shenyang Pharmaceutical University

関連論文

• Short-to Mid-Term Effects of Ovariectomy on Bone Turnover, Bone Mass and Bone Strength in Rats(Pharmacology)
• Effects of Eleven Flavonoids from the Osteoprotective Fraction of Drynaria fortunei (KUNZE) J. SM. on Osteoblastic Proliferation Using an Osteoblast-Like Cell Line
• Determination of Psoralen in Human Plasma Using Excitation-Emission Matrix Fluorescence Coupled to Second-order Calibration
• Determination of Carbendazim in Bananas by Excitation-Emission Matrix Fluorescence with Three Second-order Calibration Methods
• Simultaneous determination of cortisol and prednisolone in body fluids by using HPLC-DAD coupled with second-order calibration based on alternating trilinear decomposition
• The Influence of Ti on the Hot Ductility of Nb-bearing Steels in Simulated Continuous Casting Process
• Cardenolides from Antiaris toxicaria as Potent Selective Nur77 Modulators
• Effects of Histamine on Lipid Metabolic Disorder in Mice Loaded With Restraint Stress
• The Anti-stress Effects of Sarcandra glabra Extract on Restraint-Evoked Immunocompromise(Pharmacology)
• Coumarins from Campylotropis hirtella (FRANCH.) SCHINDL. and Their Inhibitory Activity on Prostate Specific Antigen Secreted from LNCaP Cells
• Protective Effects of Radix Rosa laevigata against Propionibacterium acnes and Lipopolysaccharide-Induced Liver Injury
• Fortuneanosides G-L, Dibenzofuran Glycosides from the Fruit of Pyracantha fortuneana
• Correlations of calcaneal QUS with pQCT measurements at distal tibia and non-weight-bearing distal radius
• Effects of Fructus Ligustri Lucidi Extract on Bone Turnover and Calcium Balance in Ovariectomized Rats(Pharmacology)
• Anti-Stress Effect of BRAND'S Essence of Chicken (BEC) on Plasma Glucose Levels in Mice Loaded with Restraint Stress
• The Protective Effect of BRAND'S Essence of Chicken (BEC) on Energy Metabolic Disorder in Mice Loaded with Restraint Stress
• Anti-Stress Effect of Oolong Tea in Women Loaded with Vigil
• Influence of Histamine in a Liver Injury Model Induced by Propionibacterium acnes and Lipopolysaccharide(Pharmacology)
• Anti-inflammatory Alkaloids from the Stems of Picrasma quassioides BENNET
• Increase in Bone Mass and Bone Strength by Sambucus williamsii HANCE in Ovariectomized Rats(Pharmacology)
• New Stilbenoids from Pholidota yunnanensis and Their Inhibitory Effects on Nitric Oxide Production
• Diterpenes from the Roots of Euphorbia kansui and Their in Vitro Effects on the Cell Division of Xenopus (2)
• pQCT bone strength index may serve as a better predictor than bone mineral density for long bone breaking strength
• Protective Effects of Radix Rosa laevigata against Propionibacterium acnes and Lipopolysaccharide-Induced Liver Injury
• Effect of Cocoa Tea (Camellia ptilophylla) Co-Administrated with Green Tea on Ambulatory Behaviors
• Flavonol Glycosides from Epimedium pubescens
• Correlation study of scanning acoustic microscope reflection coefficients and image brightness intensities of micrographed osteons
• Staining intensity of individual osteons correlates with elastic properties and degrees of mineralization
• Ultrasound detection of trypsin-treated articular cartilage : its association with cartilaginous proteoglycans assessed by histological and biochemical methods
• Alkylphenols from the Roots of Ardisia brevicaulis Induce G1 Arrest and Apoptosis through Endoplasmic Reticulum Stress Pathway in Human Non-small-cell Lung Cancer Cells
• Neuroprotective effects of genistein on dopaminergic neurons in the mice model of Parkinson's disease

もっと見る 閉じる

スポンサーリンク