A NOBEL ANIMAL MODEL FOR PERIVENTRICULAR LEUKOMALACIA TARGETING LATE OLIGODENDROCYTE PROGENITORS

概要

論文の詳細を見る
To know whether hypoxic-ischemia specific to late oligodendrocyte progenitors (preOLs: NG2-positive and O4-positive cells) can induce pathological changes similar to human periventricular leukomalacia (PVL), we first examined normal oligodendrocyte development in P2-P7 rat by counting the number of NG2-positive and O4-positive cells and then tried to make PVL model rats that exhibit similar histological changes found in human PVL with changing hypoxic-ishchemic condition. Counting of the number of developing oligodendrocytes revealed that O4-positive cells were found as early as 2 days after birth, and they gradually increased until P7, suggesting that dominant expression of preOLs was found in P2-P4. Hypoxic ishchemia by right common carotid artery occlusion (RCAO) followed by hypoxic condition (6-8% oxygen) for various duration (0-90 min) revealed that histological changes similar to human PVL in preterm delivery (partial collapse in cortical layer and corpus callo-sum, appearance of ED-1 positive cells) were found in most of P3 rats that survived in condition of RCAO with 6% oxygen for 60 min. Data indicate that new PVL model targeting to preOLs was successfully made by RCAO with 6% oxygen for 60 min, giving an animal model of PVL for the evaluation of new therapy.
名古屋市立大学の論文
2007-02-28