DEVELOPMENT OF INTRASTRIATAL HEMORRHAGE MODEL RAT WITHLOCALIZED DAMAGE NEAR INTERNAL CAPSULE

概要

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Intracerebral hemorrhage (ICH) near the internal capsule (IC) causes severe motor dysfunction even in small size of bleeding. We tried to make ICH model that has relatively small hemorrhage near the IC with relatively big motor dysfunction. Rats were injected with type IV collagenase (1.4 ul of 1-200 U/ml) into the striatum near the left IC. Behavioral function was evaluated for 7 days after the injection with five parameters: spontaneous rotation, hindlimb retraction, beam walk ability, forepaw grasp and methamphetamine-induced rotation. Hematoxylin-eosin staining revealed that 7.5 U/ml is the optimal concentration for our purpose, because higher concentrations caused too large tissue damage and lower concentrations did not induce motor dysfunction. Behavioral test also showed that the motor dysfunction in the 7.5 U/ml group was kept in relatively severe level even in 28 days after the injection. These data suggest that collagenase injection into the striatum near the IC with 1.4 ul of 7.5 U/ml induced a relative small brain damage with relatively bigger motor dysfunction, giving a candidate for good model of the analysis in pathology and new therapy.
名古屋市立大学の論文
2007-02-28