EPITOPE ANALYSIS OF THE JAPANESE CYPRESS POLLEN MAJOR ALLERGEN, CHA O 1,RECOGNIZED BY A HUMAN T CELL CLONE

概要

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One CD4^+ T cell clone with specificity for Cha o 1,a major allergen of Japanese cypress pollen, was established from peripheral blood of a patient with Japanese cypress pollinosis and epitope analysis was done using synthesized overlapping oligopeptides. We screened MHC-binding regions of Cha o1by testing HLA-DR molecules to solidphase synthetic peptides. Based on the results, we synthesized peptides, and a peptide, Cha o 1 : 202-217 (GHSDIYSDDKSMKVTV), induced a proliferative response of this clone. However, three altered peptides carrying a single amino acid substitution to alanine or serine, K214A, V215S and T216A, remarkably lost its proliferative activity. This result suggested that these amino acid residues were important for the eptiope activity. In addition, it was also suggested that a residue of ^<216>T was important for binding between the epitope peptide and antigen presenting cells (APC), because T216A did not inhibit binding between APC and a labeled original epitope peptide in an analysis using FACScan (Beckton-Dickinson, Sandiego, CA). Our results suggest a possibility of immunotherapy of Japanese cypress pollinosis through manipulating T cell responses in humans.
名古屋市立大学の論文
2002-03-25