Solid-Phase Synthesis of Crystalline Monellin, a Sweet Protein(Organic Chemistry)
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概要
- 論文の詳細を見る
The sweet protein, monellin, consists of two noncovalently associated polypeptide chains, the A chain of 44 amino acid residues and the B chain of 50 residues. The B chain was synthesized by the stepwise Fmoc solid-phase method in an overall yield of 6.2%. The synthetic B chain was combined with the synthetic A chain, which was left over from a previous work, to give monellin in a yield of 25.7%. The synthetic monellin was approximately 4000 times as sweet as sucrose, while the previously synthesized [Asn^<49>, Glu^<50>] B-chain monellin was also approximately 4000 times as sweet as sucrose. Exchanging Glu^<49> and Asn^<50> in the B chain did not affect the sweetness potency. Crystallization was performed by a vapor diffusion method.
- 社団法人日本農芸化学会の論文
- 1991-02-23
著者
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Nio Noriki
Central Research Laboratories, Ajinomoto Co., Inc.
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Ariyoshi Yasuo
Central Research Laboratories, Ajinomoto Co., Inc.
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Nio N
Ajinomoto Co. Inc. Kanagawa Jpn
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Ariyoshi Y
Ajinomoto Co. Inc. Kawasaki Jpn
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Ariyoshi Yasuo
Central Research Laboratories Ajinomoto Co. Inc
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Kohmura M
Ajinomoto Co. Inc. Kawasaki Jpn
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Kohmura Masanori
Central Research Laboratories, Ajinomoto Co., Inc.
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Kohmura Masanori
Central Research Laboratories Ajinomoto Co. Inc.
関連論文
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- Solid-phase Synthesis and Structure-Taste Relationships of Analogs of the Sweet Protein Monellin
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- Solid-Phase Synthesis of [Asn^]-, [Asn^]-, [Gln^]-, and [Asn^]Monellin, Analogues of the Sweet Protein Monellin
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- Complete Amino Acid Sequence of the Sweet Protein Monellin(Biological Chemistry)
- Solid-Phase Synthesis and Crystallization of Monellin, an Intensely Sweet Protein(Organic Chemistry)
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