Solid-Phase Synthesis of [Asn^<A16>]-, [Asn^<A22>]-, [Gln^<A25>]-, and [Asn^<A26>]Monellin, Analogues of the Sweet Protein Monellin
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概要
- 論文の詳細を見る
In an attempt to delineate the binding site(s) of monellin to the receptor by means of a structure-taste relationship, we synthesized four monellin analogues, [Asn^<A16>]-, [Asn^<A22>]-, and [Gln^<A25>]-, [Asn^<A26>]- monellin, which were 7500,750,2500,and 5500 times as sweet as sucrose on a weight basis, respectively. Among them, [Asn^<A22>] monellin and [Gln^<A25>] monellin were less sweet than monellin, and were susceptible to the HPLC conditions used. It can be concluded that Asp^<16>, Asp^<22>, Glu^<25>, and Asp^<26> residues of the A chain did not participate in binding with the receptor, since the sweet taste was not removed by replacing the amino acid residues with Asn or Gln. It can also be concluded that Asp^<22> and Glu^<25> of the A chain may have participated in intramolecular binding, as was pointed out by Kim et al., since exchanging Asp^<22> and Glu^<25> of the A chain with Asn and Gln significantly decreased the stability in solution.
- 社団法人日本農芸化学会の論文
- 1992-03-23
著者
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Nio Noriki
Central Research Laboratories, Ajinomoto Co., Inc.
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Ariyoshi Yasuo
Central Research Laboratories, Ajinomoto Co., Inc.
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Nio N
Ajinomoto Co. Inc. Kanagawa Jpn
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Ariyoshi Y
Ajinomoto Co. Inc. Kawasaki Jpn
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Ariyoshi Yasuo
Central Research Laboratories Ajinomoto Co. Inc
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Kohmura M
Ajinomoto Co. Inc. Kawasaki Jpn
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Kohmura Masanori
Central Research Laboratories, Ajinomoto Co., Inc.
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Kohmura Masanori
Central Research Laboratories Ajinomoto Co. Inc.
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