Solid-Phase Synthesis and Crystallization of [Asn^<22>, Gln^<25>, Asn^<26>]-A-Chain-[Asn^<49>, Glu^<50>]-B-Chain-Monellin, an Analogue of the Sweet Protein Monellin(Organic Chemistry)
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概要
- 論文の詳細を見る
The sweet protein monellin consists of two noncovalently associated polypeptide chains, the A chain of 44 amino acid residues and the B chain of 50 residues. The [Asn^<22>, Gin^<25>, Asn^<26>]-A chain, an analogue of the A chain, was synthesized by the stepwise Fmoc-solid-phase method in an overall yield of 13.4%. The synthetic A chain analogue was combined with the [Asn^<49>, Glu^<50>]-B chain, which was left over from a previous work, to give [Asn^<22>, Gln^<25>, Asn^<26>]-A-chain-[Asn^<49>, Glu^<50>]-B-chain-monellin in a yield of 26.2%. This synthetic monellin analogue was approximately 550 times sweeter than sucrose. Changing the carboxyl groups of Asp^<22>, Glu^<25> and Asp^<26> of the A chain to amide groups significantly decreased the sweetness potency. Crystallization was performed by a vapor diffusion method.
- 社団法人日本農芸化学会の論文
著者
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Nio Noriki
Central Research Laboratories, Ajinomoto Co., Inc.
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Ariyoshi Yasuo
Central Research Laboratories, Ajinomoto Co., Inc.
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Nio N
Ajinomoto Co. Inc. Kanagawa Jpn
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Ariyoshi Y
Ajinomoto Co. Inc. Kawasaki Jpn
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Ariyoshi Yasuo
Central Research Laboratories Ajinomoto Co. Inc
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Kohmura M
Ajinomoto Co. Inc. Kawasaki Jpn
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Kohmura Masanori
Central Research Laboratories, Ajinomoto Co., Inc.
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Kohmura Masanori
Central Research Laboratories Ajinomoto Co. Inc.
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