Immunohistochemical Observation of Antigens in Tissues Embedded in GMA-Quetol 523
スポンサーリンク
概要
- 論文の詳細を見る
Tissues embedded in GMA-Quetol 523 mixture have been used to show the localization of intracellular antigens at the light microscopic level using immunohistochemical techniques. Quetol 523 reacts with GMA (Glycol methacrylate) and becomes a integral part of the polymerized system, and the mixture shows innocuous chemical behavior in immunohistochemistry. To demonstrate this method, ovalbumin, lysozyme and ovomucoid were used as antigens. The gamma globulin of rabbit anti-serum was conjugated and purified by means of the ordinary fluorescein and enzyme-labeled antibody methods. The tissue blocks (chick oviduct) fixed in 99% ethanol containing 0.5% glacial acetic acid and formalin-alcohol were embedded in GMA-Quetol 523 mixture after dehydration in GMA-Quetol 523 mixture. Both Specific immunofluorescence and immunoperoxidase reaction products were distinctly demonstrated in the granules of serous glandular cells of the oviduct magnum. Specific reactions could be localized far more precisely than in paraffin or frozen sections without removal of the embedding matrix. The GMA-Quetol 523 mixture, therefore, seemed to be a promising embedding medium for immunohistochemical studies.
- 東海大学の論文
著者
-
Suzuki Toshio
Department Of Agronomy Faculty Of Agriculture Tohoku University
-
Nagato Yasukazu
Department Of Anatomy School Of Medicine Tokai University
関連論文
- Androgen Suppresses Corticotropin-Induced Increase in Plasma Cortisol Level but Enhances The Increase in Plasma Aldosterone Level in Goats(Physiology)
- Numerical Simulation of Peritectic Reaction in Fe-C Alloy Using a Multi-phase-field Model
- Phase-field modeling of rapid solidification in small alloy droplets
- Ostwald Ripening Analysis Using Phase-Field Model
- Numerical Prediction of the Secondary Dendrite Arm Spacing Using a Phase-feild Model
- Phase-field Model for Solidification of Ternary Alloys
- Numerical Simulation of the Critical Velocity for Particle Pushing/Engulfment Transition in Fe-C alloys Using a Phase-field Model
- Numerical Simulation of Interface Shape around an Insoluble Particle for Fe-C Alloys Using a Phase-field Model
- Design, Production, and Characterization of Recombinant Neocarzinostatin Apoprotein in Escherichia coli
- Identification of cell proliferation-associated epitope on CD98 oncoprotein using phage display random peptide library
- Molecular Structural and Functional Characterization of Tumor Suppressive Anti-ErbB-2 Monoclonal Antibody by Phage Display System
- Phage Display Cloning and Characterization of Monoclonal Antibody Genes and Recombinant Fab Fragment against the CD98 Oncoprotein
- 2-Hydroxyphenyl-1, 3-dimethylbenzimidazolines. Formal Two Hydrogen Atom-donors for Photoinduced Electron Transfer Reactions
- Recent Advances in the Phase-field Model for Solidification
- Sarcolemmal ATP-sensitive K^+ channels and cardiovascular function
- Numerical Simulation of Peritectic Reaction Using a Multi-phase-field Model
- Effects of lipo-prostaglandin E_1 on blood flow and oxygen pressure in lumbo-sacral nerve roots
- No Association of the G1287A Polymorphism in the Norepinephrine Transporter Gene and Susceptibility to Major Depressive Disorder in a Japanese Population(Biopharmacy)
- Genetic Polymorphism of C452T (T127I) in Humanγ-Glutamyl Hydrolase in a Japanese Population(Biopharmacy)
- Immunoliposome-Mediated Gene Transfer into Cultured Myotubes
- Examination of Local Anti-inflammatory Activities of New Steroids, Hemisuccinyl Methyl Glycolates
- Application of Sweet Potato Fiber to Skin Wound in Rat
- Localization of Periodate-Schiff Reactive Glycosaminoglycans in Semi-thin Sections Embedded in GMA-Quetol 523-MMA : Application of a Method for Correlative Light and Electron Microscopy of Identical Sites
- Chrono and clinical pharmacokinetic study of tacrolimus in continuous intravenous administration
- Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury
- Analysis of Pion Photoproduction on Nucleons below 500 MeV
- The Production Asymmetry in γ+n⇾π^-+p Process with Polarized Photons
- Characterization of a Shiga Toxin 1-Neutralizing Recombinant Fab Fragment Isolated by Phage Display System
- Different Binding Property of Verotoxin-1 and Verotoxin-2 Against Their Glycolipid Receptor, Globotriaosylceramide
- A Rapid and Simple Detection of Genetic Defects Responsible for the Phenotypic Polymorphism of Cytochrome P450 2C19
- Recombinant Human Monoclonal Fab Fragments against Rotavirus from Phage Display Combinatorial Libraries
- S-C-7. Clinical and Experimental Studies on Whiplash Injury
- Calculation model of CO_2 emission by using a population balance model
- Sum Rules of the Multiple Giant Resonance States(Nuclear Physics)
- A Sum-Rule Constraint on the Soft Dipole Mode
- Cost-Effective Unbiased Straight-Line Fitting to Multi-Viewpoint Range Data (Special Section of Selected Papers from the 9th Karuizawa Workshop on Circuits and Systems)
- Spectra of Neutrons Emitted from Excited/Ground States of ^24,25O
- Spectra of Neutrons Emitted from Excited/Ground States of ^O
- Numerical Simulation of Switching Behavior in Cu/Cu_2S Nanometer-Scale Switch
- A Molecular Dynamics Study of the Energy and Structure of the Symmetric Tilt Boundary of Iron
- Magnetic properties of light neutron-rich nuclei(International Workshop on Nuclear Structure-New Pictures in the Extended Isospin Space(NS07)-)
- Dynamical Effects beyond Mean Field in Drip Line Nuclei
- Syntheses of four possible enantioners of 2, 3-desubstituted-γ-butyrolactones from a single progenitor, (R) -2, 3-O-isopropylideneglyceraldehyde
- STUDY ON RE-ESTABLISHMENT OF OVULATION AFTER TERMINATION OF SEX-STEROIDAL TREATMENT : COMPARED WITH RE-APPEARANCE OF OVULATION AFTER ABORTION AND PREMATURE DELIVERY
- Orientation Relationship in Fcc-Bcc Phase Transformation Kinetics of Iron : a Molecular Dynamics Study
- A Molecular Dynamics Study of Thermodynamic and Kinetic Properties of Solid-Liquid Interface for Bcc Iron
- Electromagnetic Energy Shift of Nucleus between Conducting Plates : Nuclear Physics
- Numerical Simulation of Isothermal Dendritic Growth by Phase-field Model
- Comparison of Calculated Values with Measured Values on the Amount of TRU and FP Nuclides Accumulated in Gadolinium Bearing PWR Spent Fuels
- Light and Electron Microscopic Observations of Glycogen in Semi-thin Sections Employing GMA, Quetol 523 and Methyl Methacrylate
- DESIGN AND SYNTHESIS OF COLON-SPECIFIC PRO-ANTEDRUGS, 20-GLUCOPYRANOSYLOXY METHYLPREDNISOLONATES, FOR ORAL TREATMENT OF ULCERATIVE COLITIS AND THEIR METABOLISM
- Simultaneous determination of lansoprazole enantiomers and their metabolites in plasma by liquid chromatography with solid-phase extraction
- IMMUNOHISTOCHEMICAL INVESTIGATION OF AMINOPEPTIDASE
- IMMUNOHISTOCHEMICAL STUDY ON ARGINASE IN THE BOVIEN LIVER
- IMMUNOHISTOCHEMICAL INVESTIGATION ON THE SEROUS GLAND OF CHICK OVIDUCT : I. ON THE LOCALIZATION OF OVALBUMIN
- LOCALIZATION OF DIAMINE OXIDASE AND D-AMINO ACID OXIDASE IN KIDNEY, DEMONSTRATED BY MEANS OF IMMUNOHISTOCHEMICAL METHOD
- Demosntration of Xanthine Oxidase on the Electron Microscopic Level
- Electron Microscopic Histochemistry of Succinate-ferricyanide Reductase in the Dark Cell of the Chick Liver
- Characterization of debonding energy release rate of FRP sheets bonded on mortar and concrete
- LOCALIZATION OF PAROTIN IN BOVINE PAROTID GLAND, DEMONSTRATED BY THE IMMUNOHISTOCHEMICAL METHOD
- Effects of GABAergic Drugs on the Recovery of Reflex Potentials after Spinal Cord Ischemia in Cats
- Pulmonary Shunting during Venovenous Bypass
- Role of the Landau-Migdal Parameters with the Pseudovector and Tensor Couplings in Relativistic Nuclear Models : The Quenching of the Gamow-Teller Strength(Nuclear Physics)
- PAS Staining of Eosinophils in Semi-thin Sections of Bone Marrow Embedded in Glycol Methacrylate
- The Continuity Equation of RPA
- High Efficiency Gas Cherenkov Counter
- Examination on Biological Activities and Fates of New Steroids, Steroid-17-yl Methyl Glycolate Derivatives
- Immunohistochemical Observation of Antigens in Tissues Embedded in GMA-Quetol 523
- STUDIES ON INFECTION AND THE MULTIPLICATION OF PLANT VIRUSES (III) RELATIONSHIP BETWEEN THE CELL NUCLELTS AND CUCUMBER MOSAIC VIRLTS REPLICATION
- Faceted Crystal Growth of Silicon from Undercooled Melt of Si-20mass%Ni Alloy
- Observation on Backscattered Electron Image (BEI) of a Scanning Electron Microscope (SEM) in Semi-thin Sections Prepared for Light Microscopy
- Preparation of 1- to 2-Micron Sections of Plastic Embedded Tissue for Enzyme Histochemistry
- Immunohistochemical Study of Aminopeptidase by Mean of Fluorescent Antibody Technique
- Three-dimensional Phase-field Simulation of Free Dendrite Growth of Iron
- Li^6 and Electron Scattering
- Recognizing tautology by a deterministic algorithm whose while-loop's execution time is bounded by forcing
- Accuracy and Computational Efficiency of Phase-field Models
- Free Dendrite Growth of Fe-0.5mass%C Alloy : Three-dimensional Phase-field Simulation and LKT Model
- Phase-Field Simulation of Free Dendrite Growth of Aluminum-4.5mass%Copper Alloy
- Halo Structure of ^Li and Gamow-Teller Transitions to ^Be
- Numerical Simulation of Initial Microstructure Evolution of Fe-C Alloys Using a Phase-field Model
- Inverse Iteration Method with Multiple Cyclotomically Shifted Parameters
- Flavonoids isolated from Allium cepa L. var. agrogatum Don(Natural Medicine Note)
- Immunohistochemical study of chick oviduct
- A Molecular Dynamics Study of Bidirectional Phase Transformation between bcc and fcc Iron
- Estimation of Solid-liquid Interfacial Energy from Gibbs-Thomson Effect : A Molecular Dynamics Study
- Gamow-Teller Strengths in Ni Isotopes and Nuclear Weak Processes(YKIS2011 papers, Frontier Issues in Physics of Exotic Nuclei)
- Role of the Landau-Migdal Parameters with the Pseudovector and Tensor Couplings in Relativistic Nuclear Models : The Quenching of the Gamow-Teller Strength(Nuclear Physics)
- Zeta potentials of metal oxides coated with titanate coupling agents in aqueous solution.
- The Solvent Effect on the Electron-Transfer Reactions of Cobalt(III) Complexes. The Reduction of the cis-Chloro-2-aminoethanolbis(ethylenediamine)cobalt(III) Ion by Iron(II) in Aqueous Solutions of Organic Solvents
- An immunohistochemical study of lysozyme in the human nasal mucosa.
- Effects of Naloxone ad Levallorphan on the Spinal Cord Reflex Potentials under the Spinal Ischemic Condition in Cats.
- Gamow-Teller Strengths in Ni Isotopes and Nuclear Weak Processes