鉛の個人暴露指標としての赤血球のALAD活性阻害率

概要

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As the δ-aminolevulinic acid dehydratase (ALAD) activity in erythrocytes is decreased by lead exposure, we considered that a net reduction of ALAD activity by lead in blood should be the difference between the activity fully activated with zinc (Zn^<2+>) and dithiothreitol (DTT) and that without activation. The optimal condition of activation of ALAD was found by addition of 0.25 mM of Zn^<2+> and 10 mM of DTT in the reaction mixture. Judging from our previous results that the amount of inhibition of ALAD activity can be represented as the rate of inhibition and is closely correlated with the dose of lead administered to rabbits, the inhibition rate of ALAD activity and lead content in blood (Pb-B) of lead workers were measured. The scatter diagram obtained from the inhibition rate and lead content in blood has two groups being divided at 50 μg/ml of Pb-B. In one group less than 50 μg/100 ml of Pb-B, the inhibition rate has been closely related to Pb-B., the regression equation being Y=1.82X+11.7, and the correlation coefficient +0.926. In another group more than 50 μg/100 ml of Pb-B the inhibition rate remained constant at the 90% level. Measurement of the inhibition rate suggests to have practical validity for monitoring lead exposure in workers, and by means of a nomograph lead content in blood can be estimated from the inhibition rate.
社団法人日本産業衛生学会の論文
1983-09-20