The Identification of DNA Binding Factors Specific for as-1-like Sequences in Auxin-Responsive Regions of parA, parB and parC
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概要
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We have identified the auxin-responsive region (AuxRR) of the parA promoter; it is derived from a gene that is induced by auxin in tobacco mesophyll protoplasts. By analyses of gain-of-function and point mutations in transgenic tobacco plants, we showed that an as-1-like sequence was required, but not alone sufficient, for auxin responsiveness of the parA promoter, as has been also shown for the parC promoter in a recent study. A gel mobility shift assay revealed that the as-1-like sequence of the parA promoter bound specifically to the as-1 binding nuclear factor, ASF-1, while the as-1-like sequence of the parC promoter bound specifically to a novel nuclear factor named ALF-1 (a__-s-1-l__-ike sequence binding f__-actor-1). Since these bindings were correlated with auxin-induced activation of transcription, those results suggest that ALF-1 is a new nuclear factor involved in auxin responsiveness and that it is distinct from ASF-1. Furthermore, we found that an as-1-like sequence in the AuxRR of parB bound specifically to a tobacco nuclear factor, named ALF-2, which differed from ASF-1 and ALF-1. These results suggest that the auxin responsiveness of the auxin-inducible genes parA, parB and parC are regulated by different mechanisms, even though the cis-acting elements look similar.
- 日本植物生理学会の論文
著者
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Nagata Toshiyuki
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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SAKAI Tatsuya
Department of General Medicine and Clinical Epidemiology, Kyoto University Graduate School of Medici
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Sakai Tatsuya
Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G
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Takahashi Y
Okayama Univ. Okayama Jpn
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Takahashi Yohsuke
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Sakai Tatsuya
Department Of Biological Sciences Graduate School Of Science University Of Tokyo:(present Address)de
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Nagata T
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Nagata Toshiyuki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Nagata Toshiyuki
Dept. Of Biological Sciences Graduate School Of Science. Univ. Of Tokyo
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Sakai Tatsuya
Department Of Applied Chemistry Faculty Of Science And Engineering Chuo University
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