Introduction of Functional RNA into Plant Protoplasts by Electroporation

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概要

• 論文の詳細を見る

• A simple apparatus was constructed for producing electric discharge of varying intensities between two electrodes in a spectrophotometer cuvette. This apparatus was used to study the conditions for efficient introduction of functional RNA into plant protoplasts using RNAs of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Electroporation under optimal conditions resulted in the production of TMV and CMV in 80% of the protoplasts from tobacco cell line BY2. Infection by TMV-RNA occurred in 60% of Vinca rosea suspension culture protoplasts and in 40% of tobacco mesophyll protoplasts. Electroporation in the presence of TMV and CMV particles also resulted in infection, suggesting that pores larger than 30 nm are formed. The advantages and uses of the electrical method for introduction of functional RNA are discussed.

• 日本植物生理学会の論文

著者

• Okada K

  Department Of Botany Faculty Of Science Kyoto University

• Nagata Toshiyuki

  Department of Biological Sciences, Graduate School of Science, University of Tokyo

• OKADA KAZUYA

  Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences

• Okada Kazuya

  Department Of Biology Faculty Of Science Nagoya University

• Okada Kiyotaka

  Department Of Botany Faculty Of Science Kyoto University

• Takebe Itaru

  Department of Biology, Faculty of Science, Nagoya University

• Nagata T

  Department Of Biological Sciences Graduate School Of Science University Of Tokyo

• Nagata Toshiyuki

  Department Of Biological Sciences Graduate School Of Science The University Of Tokyo

• Nagata Toshiyuki

  Dept. Of Biological Sciences Graduate School Of Science. Univ. Of Tokyo

• Takebe I

  Nagoya Univ. Nagoya Jpn

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