Introduction of Functional RNA into Plant Protoplasts by Electroporation
スポンサーリンク
概要
- 論文の詳細を見る
A simple apparatus was constructed for producing electric discharge of varying intensities between two electrodes in a spectrophotometer cuvette. This apparatus was used to study the conditions for efficient introduction of functional RNA into plant protoplasts using RNAs of tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV). Electroporation under optimal conditions resulted in the production of TMV and CMV in 80% of the protoplasts from tobacco cell line BY2. Infection by TMV-RNA occurred in 60% of Vinca rosea suspension culture protoplasts and in 40% of tobacco mesophyll protoplasts. Electroporation in the presence of TMV and CMV particles also resulted in infection, suggesting that pores larger than 30 nm are formed. The advantages and uses of the electrical method for introduction of functional RNA are discussed.
- 日本植物生理学会の論文
著者
-
Okada K
Department Of Botany Faculty Of Science Kyoto University
-
Nagata Toshiyuki
Department of Biological Sciences, Graduate School of Science, University of Tokyo
-
OKADA KAZUYA
Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences
-
Okada Kazuya
Department Of Biology Faculty Of Science Nagoya University
-
Okada Kiyotaka
Department Of Botany Faculty Of Science Kyoto University
-
Takebe Itaru
Department of Biology, Faculty of Science, Nagoya University
-
Nagata T
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
-
Nagata Toshiyuki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
-
Nagata Toshiyuki
Dept. Of Biological Sciences Graduate School Of Science. Univ. Of Tokyo
-
Takebe I
Nagoya Univ. Nagoya Jpn
関連論文
- Comparative Analysis of Plastid Gene Expression in Tobacco Chloroplasts and Proplastids : Relationship between Transcription and Transcript Accumulation
- Treatment of bleeding stomal varices by balloon-occluded retrograde transvenous obliteration
- Studies on Interactions between Traditional Herbal and Western Medicines.V. Effects of Sho-saiko-to (Xiao-Cai-hu-Tang) on the Pharmacokinetics of Carbamazepine in Rats
- Identification of Lysosome-like Structures in a Unicellular Red Alga Cyanidioschyzon merolae
- A Serine/Threonine Protein Kinase Gene Isolated by an in vivo Binding Procedure Using the Arabidopsis Floral Homeotic Gene Product, AGAMOUS
- Regulation of CAPRICE Transcription by MYB Proteins for Root Epidermis Differentiation in Arabidopsis
- The analysis of CPC gene in root hair formation in Arabidopsis thaliana
- Regulatory Systems of Root Patterning
- An Arabidopsis ACT2 Dominant-Negative Mutation, which Disturbs F-actin Polymerization, Reveals its Distinctive Function in Root Development
- Aortoenteric Fistula After Endovascular Stent Grafting for an Abdominal Aortic Aneurysm : Report of a Case
- A Cytokinin-Repressed Gene in Cucumber for a bHLH Protein Homologue is Regulated by Light
- PROPERTIES OF THE GENE FOR A bHLH PROTEIN REPRESSED RAPIDLY AFTER APPLICATION OF CYTOKININ
- THE ANALYSES OF THE GENE FOR A TRANSCRIPTIONAL ACTIVATOR REPRESSED RAPIDLY AFTER APPLICATION OF CYTOKININ
- Co-localization of Urokinase and its Receptor on Established Human Umbilical Vein Endothelial Cell
- The Role of the Pericellular Fibrinolytic System in Angiogenesis
- Biocompatible Block Copolymers Composed of Polydimethylsiloxane and Poly[(2-methacryloyloxy)ethyl phosphorylcholine] Segments
- Surface Modified Poly(methyl methacrylate) Microspheres with the O-Methacryloyl-L-serine Moiety
- Oblique Cell Plate Formation in Tobacco BY-2 Cells Originates in Double Preprophase Bands
- Studies on Plastid-Nuclei (Nucleoids) in Nicotiana tabacum L. I. Isolation of Proplastid-Nuclei from Cultured Cells and Identification of Proplastid-Nuclear Proteins
- The Identification of DNA Binding Factors Specific for as-1-like Sequences in Auxin-Responsive Regions of parA, parB and parC
- ANALYSIS OF THE PROMOTERS OF AUXIN-INDUCIBLE PAR GENES
- Analysis of the Promoter of the Auxin-Inducible Gene, parC, of Tobacco
- Multiplication and Differentiation of Plastid Nucleoids during Development of Chloroplasts and Etioplasts from Proplastids in Triticum aestivum
- Fate of Nascent Microtubules Organized at the M/G_1 Interface, as Visualized by Synchronized Tobacco BY-2 Cells Stably Expressing GFP-Tubulin : Time-Sequence Observations of the Reorganization of Cortical Microtubules in Living Plant Cells
- pnp-Type GaAs Inversion-Base Bipolar Transistor (pnp-type GaAs IBT)
- Integration of a GaAs SISFET and GaAs Inversion-Base Bipolar Transistor : Special Section : Solid State Devices and Materials 2 : III-V Compound Semiconductors Devices and Materials
- Isolation and Characterization of a Cytokinin Up-Regulated Gene from Tobacco Mesophyll Protoplasts
- FUNCTIONAL ANALYSIS OF A bZIP TRANSCRIPTIONAL FACTOR, RSG THAT MIGHT BE INVOLVED IN INTERNODE GROWTH
- ANALYSIS OF A HOMOLOGUE OF arcA, AN AUXIN-PREGULATED GENE, IN YEAST
- FUNCTIONAL INTERPRETATION OF AN AUXIN-REGULATED GENE, ARC A
- The Mode of Expression and Promoter Analysis of the arcA Gene, an Auxin-Regulated Gene in Tobacco BY-2 Cells
- IDENTIFICATION OF FROTEIN THAT-INTERACTS WITH THE PRODUCT OF AUXINREGULATED GENE arcA.
- Auxin-Regulated Genes : GROWTH AND DEVELOPMENT : GENE STRUCTURE AND EXPRESSION
- ANALYSIS OF THE PRODUCT OF ANAUXIN-REGULATED GENE arcA
- Functions and Modulations of Auxin-Regulated Genes
- Introduction of Functional RNA into Plant Protoplasts by Electroporation
- VAJ/GFA1/CLO is Involved in the Directional Control of Floral Organ Growth
- Granular Cell Tumor of the Breast: A Case Report Describing Dynamic MR Mammography
- The Arabidopsis FLAKY POLLEN1 Gene Encodes a 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase Required for Development of Tapetum-Specific Organelles and Fertility of Pollen Grains
- An attemt to develop the in vitro assay system of the organization of plant microtubules
- MICROTUBULE ORGANIZING CENTER(MTOC)SITES IN TOBACCO BY-2 CELLS DURING CELL CYCLE PROGRESSION
- ANALYSIS OF MOLECULES INVOLVED IN THEORGANIZATION OF MICROTUBULES IN TOBACCO BY-2 CELLS
- Phosphate as Limiting Factor for the Cell Division of Tobacco BY-2 Cells
- PHOSPHATE REGULATED GENES FROM TOBACCO BY-2 CELLS
- MAA3 (MAGATAMA3) Helicase Gene is Required for Female Gametophyte Development and Pollen Tube Guidance in Arabidopsis thaliana
- Studies on Plastid-Nuclei (Nucleoids) in Nicotiana tabacum L. III. Isolation of Chloroplast-Nuclei from Mesophyll Protoplasts and Identification of Chloroplast DNA-Binding Proteins
- Acceleration of Chloroplast Division in Somatic Cell Hybrids between Mesophyll and Cell Culture Protoplasts
- Studies on Plastid-Nuclei (Nucleoids) in Nicotiana tabacum L. IV. Association of Chloroplast-DNA with Proteins at Several Specific Sites in Isolated Chloroplast-Nuclei
- Studies on Plastid-Nuclei (Nucleoids) in Nicotiana tabacum L. II. Disassembly and Reassembly of Proplastid-Nuclei Isolated from Cultured Cells
- The Possible Involvement of a Phosphate-Induced Transcription Factor Encoded by Phi-2 Gene from Tobacco in ABA-signaling Pathways
- GaAs Inversion-Base Bipolar Transistor (GaAs IBT) with Graded Emitter Barrier : Semiconductors and Semiconductor Devices
- CHARACTERIZATION OF A CELL DIVISION FACTOR THAT IS SECRETED FROM A HABITUATED TOBACCO CELL LINE 2B-13
- PHOSPHATE AS A LIMITING FACTOR FOR PROLIFERATIVE GROWTH OF CULTURED TOBACCO BY-2 CELLS
- A role of internal layer upon the heterophyllous leaf changes of Ludwigia arcuata
- Characterization of Sugar Moieties of a Cell Division Factor from Tobacco 2B-13 Cells
- Differential Expression of an Auxin-Regulated Gene, parC, and a Novel Related Gene, C-7, from Tobacco Mesophyll Protoplasts in Response to External Stimuli and in Plant Tissues : GROWTH AND DEVELOPMENT : GENE STRUCTURE AND EXPRESSION
- Arabidopsis RPT2α Encoding the 26S Proteasome Subunit is Required for Various Aspects of Root Meristem Maintenance, and Regulates Gametogenesis Redundantly with its Homolog, RPT2b
- Caffeine-Induced Uncoupling of Mitosis from DNA Replication in Tobacco By-2 Cells