The Mode of Expression and Promoter Analysis of the arcA Gene, an Auxin-Regulated Gene in Tobacco BY-2 Cells
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概要
- 論文の詳細を見る
The arcA, a member of the G protein β-subunit fami-ly, was isolated from tobacco BY-2 cells as an auxin-responsive gene. Characterization of arcA, which should help to elucidate the function of the gene product in the plant cells, was performed with emphasis on the mode of expression and the analysis of its promoter. Accumulation of the arcA message was detected only after treatments with auxins and not after treatments with other phytohormones or CdCl_2, implying that responsiveness of arcA was exclusive to auxin. The putative arcA promoter region was fused to a reporter gene for β-glucuronidase (GUS), and transient expression was analyzed in tobacco BY-2 cells. Two series of arcA promoter/GUS chimeric genes were constructed. One consisted of a set of 5' nested deletions of the arcA promoter connected to the gene for GUS and the other consisted of a variety of the arcA promoter fragments fused to a minimal promoter-GUS construct. The results indicated that the promoter sequence covering four sets of direct repeats (-562 to -167) was necessary for the sufficient response of arcA promoter to auxin in BY-2 cells. Moreover, irrespective of auxin treatment, elevated activity of GUS driven by this promoter fragment was detected, a result that implies that this region behaves an enhancer in BY-2 cells.
- 日本植物生理学会の論文
著者
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石田 さらみ
東大院・理・生物科学
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Nagata Toshiyuki
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Takahashi Yohsuke
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Ishida Sarahmi
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Ishida Sarahmi
Dept. Biol. Sci. Grad. School Of Sci. Univ.
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Takahashi Yohsuke
Dept. Biol. Sci. Grad. School Of Sci. Univ.
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Nagata T
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Nagata Toshiyuki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Nagata Toshiyuki
Dept. Of Biological Sciences Graduate School Of Science. Univ. Of Tokyo
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Takahashi Yohsuke
Dept. Biological Sci. Grad. Sch. Sci. Univ.
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