20. bZIP型転写因子RSGによるジベレリン生合成フィードバック制御機構の解析(口頭発表)

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概要

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• RSG is a bZIP transcriptional activator that regulates endogenous amounts of gibberellins. The endogenous level of GA decreased in the transgenic tobacco expressed of dominant negative form of RSG (dnRSG). The transgenic tobacco exhibited dwarf phenotypes.We investigated the expression levels of six GA biosynthetic genes. The expression of ent-kaurene oxidase is decreased in the transgenic tobaccos. RSG regulates GA biosynthesis through the transcriptional control of ent -kaurene oxidase gene. When endogenous level of GA decrease, the expression level of GA20-oxidase gene and GA3-oxidase gene increased by feedback regulation. Interestingly, in dnRSG plants, though decrease of endogenous GA, did not occur the feedback regulation of GA20-oxidase. dnRSG inhibits the feedback regulation of the GA20-oxidase (Ntc12) gene. GAs regulates intracellular localization of RSG. To search where is necessary to feedback regulation in Ntc12 promoter, we produced transgenic tobacco that GUS gene fused to Ntc12 promoter. We identified a cis element that is important of GA feedback regulation. RSG binds this element directly. The point mutation of this element in Ntc12 promoter is disrupting the feedback regulation. Moreover ChIP analysis suggests that RSG bind to Ntc12 promoter dependent on endogenous GA level.These results indicate that RSG regulates feedback regulation of GA in Ntc12 promoter.

• 2007-10-05

著者

• 深澤 壽太郎

  理研・植物科学研究センター

• 高橋 陽介

  広島大

• 高橋 陽介

  広島大・理・生物科学

• 石田 さらみ

  東大院・理・生物科学

• Ishida Sarahmi

  Dept. Biol. Sci. Grad. School Of Sci. Univ.

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