Isolation and Characterization of a Cytokinin Up-Regulated Gene from Tobacco Mesophyll Protoplasts
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概要
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We have isolated a cytokinin up-regulated cDNA clone, H13, from an early stage of cultured tobacco mesophyll protoplasts by a differential display method. The expression of this gene was specifically induced by natural and synthetic cytokinins including N-(2-chloro-4-pyridyl)-N'-phenylurea(4PU30), a diphenylurea-type cytokinin, althought the simultaneous presence of auxin was also required. It seems that the preceding treatment of the tobacco mesophyll protoplasts by auxin is necessary for the gene to respond to cytokinin. The addition of a cytokinin antagonist, compound 182, which suppressed the induction of cell division in tobacco mesophyll protoplasts, completely abolished the expression of this gene. Thougt the predicted gene product of H13 did not suggest us any sequences of defined functions, two domains of the predicted sequence had significant homology to several reported sequences in the data base. The gene product of H13 is proposed to have a role in regeneration cell wall in cultured protoplasts, since a cDNA clone E6, from cotton fiber cells, which has the most closely related structure to H13, has been isolated from cells which showed active cellulose synthesis. This supposition is supported by the evidenece that in the absence of cytokinin, cell wall regeneration was significantly suppressed, resulting in failure of the induction of cell division. Thus, the gene product of H13 is supposed to have a role in regenerating cell walls and facilitating the progression of the cell cycle, resulting in the sustained cell division of tobacco mesophyll protoplasts.
- 日本植物生理学会の論文
著者
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Saito Tsutomu
Department of Internal Medicine, Nippon Medical School
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石田 さらみ
東大院・理・生物科学
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Nagata Toshiyuki
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Saito Tsutomu
Department Of Internal Medicine Nippon Medical School
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Takahashi Yohsuke
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Iwahara Mizuho
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Ishida Sarahmi
Department of Biological Sciences, Graduate School of Science, University of Tokyo
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Iwahara Mizuho
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Ishida Sarahmi
Dept. Biol. Sci. Grad. School Of Sci. Univ.
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Takahashi Yohsuke
Dept. Biol. Sci. Grad. School Of Sci. Univ.
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Nagata T
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Nagata Toshiyuki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Nagata Toshiyuki
Dept. Of Biological Sciences Graduate School Of Science. Univ. Of Tokyo
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Takahashi Yohsuke
Dept. Biological Sci. Grad. Sch. Sci. Univ.
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Saito Tsutomu
Department Of Applied Physics Faculty Of Engineering Tohoku University
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Saito Tsutomu
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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