Response of Glutamine Synthetase and Glutamate Synthase Isoforms to Nitrogen Sources in Rice Cell Cultures
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概要
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As a model system with no photorespiration and no long distance transport, rice cell cultures (Oryza sativa L. cv Sasanishiki) were used to investigate the effect of nitrogen sources on the levels of isoforms of glutamine synthetase (GS) and glutamate synthase (GOGAT). Isoforms of GS and GOGAT were analyzed by immunoblotting methods and their activities in early growth phase of the cells. Cytosolic type GS (41 kDa subunit) and NADH-COGAT were the major isoforms in the rice cells grown in normal R-2 medium. However, contents of plastid type GS (44 kDa subunit) and Fd-GOGAT increased in response to NO^-_3 supply. NADH-GOGAT activity also in-creased following the supply of NO^-_3. In vitro translated products from poly(A)^+RNA prepared from the cells showed that the precursor of plastid type GS (49 kDa) was detected at 48 h after the inoculation. Supply of NH^+_4 resulted in an increase in NADH-GOGAT activity but had no effect on the levels of Fd-GOGAT, of polypeptides of the plastid type GS or of the corresponding mRNAs.
著者
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Yamaya Tomoyuki
Laboratory Of Plant Cell Engineering Department Of Agricultural Chemistry Faculty Of Agriculture Toh
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Yamaya Tomoyuki
Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G
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Yoneyama Tadakatsu
Department Of Applied Biological Chemistry The University Of Tokyo
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Hayakawa Toshihiko
Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological
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Ojima Kunihiko
Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological
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KAMACHI Kazunari
Laboratory of Plant Nutrition, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku
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Kamachi Kazunari
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Kamachi K
Department Of Bacterial Pathogenesis And Infection Control National Institute Of Infectious Diseases
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Yoneyama T
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Ojima K
Laboratory Of Plant Nutrition Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku Uni
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Ojima Kunihiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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Hayakawa Toshihiko
Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G
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Hayakawa Toshihiko
Laboratory Of Plant Nutrition Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku Uni
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Oikawa Mizushiro
Laboratory of Plant Nutrition, Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku
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Oikawa M
Tohoku Univ. Sendai
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Yoneyama Tadakatsu
Univ. Tokyo Tokyo Jpn
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Oikawa Mizushiro
Laboratory Of Plant Nutrition Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku Uni
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