CHARACTERIZATION OF TWO TOBACCO CELL LINES SELECTED TO GROW IN THE PRESENCE OF EITHER IONIC AI OR INSOLUBLE Al-PHOSPHATE
スポンサーリンク
概要
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A tobacco cell line tolerant to ionic Al (AlT) was selected at a frequency of 10^<-6> to 10^<-7> by plating the wild-type cells on agar medium containing 2.5 m_M AlCl_3 and 0.1 m_M phosphate at pH 4.0 Another cell line which is able to grow with insoluble Al-phosphate as a sole source of phosphate (IPU) was also selected by subculturing the wild-type cells for more than 10 passages in suspension culture in the presence of 2m_M phosphate and 4m_M AlCl_3 at pH 5.0. The IPU cell line also tolerated ionic Al but the AlT line showed poor growth with insoluble Al-phosphate as a sole source of phosphate. Both cell lines excreted citrate into the media. The rate of citrate excretion by the IPU cell line, on a fresh weight basis, was higher than that by the AlT and wild-type cell lines.
- 社団法人日本土壌肥料学会の論文
著者
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Yamaya Tomoyuki
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Yamaya T
Tohoku Univ. Sendai Jpn
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Yamaya T
Institute For Agricultural And Biological Sciences. Okayama University
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KOYAMA Hiroyuki
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University
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Yamaya Tomoyuki
Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G
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Ojima K
Tohoku Univ. Sendai Jpn
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Ojima Kunihiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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Suzuki R
Yamagata Univ. Yamagata Jpn
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Koyama H
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
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Koyama Hiroyuki
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
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SUZUKI Rika
Laboratory of Plant Nutrition , Department of Agricultural Chemistry, Faculty of Agriculture, Tohoku
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Koyama Hiroyuki
Laboratory Of Plant Cell Technology Department Of Biotechnology Faculty Of Agriculture Gifu Universi
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Koyama Hiroyuki
Laboratory Of Animal Physiology And Functional Anatomy Graduate School Of Agriculture Kyoto University
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