Simple Identification of Transgenic Arabidopsis Plants Carrying a Single Copy of the Integrated Gene
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概要
- 論文の詳細を見る
Transgenic Arabidopsis thaliana plants carrying a single copy of integrated DNA can be identified by single-step genomic polymerase chain reaction. The reaction employs two sets of primer pairs with the same melting temperature that amplify the amplicons derived from the integrated T-DNA together with those from an endogenous single-copy gene as a reference. When the band intensity ratio is one, this means that the transgenic plants are carrying a single copy of the integrated gene per haploid.
- 社団法人 日本農芸化学会の論文
- 2006-07-23
著者
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ZHAO Cheng-Ri
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University
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KOYAMA Hiroyuki
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University
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KAWAZU TETSU
Forestry Research Institute, Oji Paper Co. Ltd.
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Kihara Tomonori
Forestry Research Institute Oji Paper
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Takita Eiji
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Kawazu Tetsu
Forestry Research Institute Oji Paper
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Zhao Cheng-ri
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
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KOBAYASHI Yuriko
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University
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Kobayashi Yuriko
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
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Koyama H
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
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Koyama Hiroyuki
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
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Koyama Hiroyuki
Laboratory Of Plant Cell Technology Department Of Biotechnology Faculty Of Agriculture Gifu Universi
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Koyama Hiroyuki
Laboratory Of Animal Physiology And Functional Anatomy Graduate School Of Agriculture Kyoto University
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