Characteristics of Aluminum-Phosphate-Adapted Carrot Cells: Uptake and Utilization of the Phosphate
スポンサーリンク
概要
- 論文の詳細を見る
To elucidate the mechanism responsible for the superior growth of a selected line of carrot cells (Daucus carota L. cv MS Yonsun) in medium that contained AlPO_4, kinetic studies of the up-take of phosphate and the efficiency of utilization of phosphate were performed with the selected cells and the wild-type cells. When the two cell lines were grown in a medium with adequate soluble phosphate (2 mM), there was no difference between their growth rates. Rates of increase in fresh weight as a function of increasing concentration of phosphate in the medium were also identical between the cell lines, indicating that the efficiency of utilization of phosphate by the selected cell line was similar to that by the wild-type cells. However, rate of uptake of phosphate by the selected cells under phosphate limited conditions (20 μM NaH_2PO_4 at pH 5.6) was about 5-fold higher than that by the wild-type cells. Apparent K_m values for the uptake of phosphate were calculated to be 13.6 and 9.1 pM for the selected and the wild-type cells, respectively. The V_<max> value was estimated to be 88.8 nmol per g fresh weight per min for the selected cells and 28.2 for the wild-type cells. Thus, the selected cells has an enhanced system for uptake of phosphate whereby there was an increase in the rate of the uptake without any dramatic change in the affinity for phosphate ions.
- 日本植物生理学会の論文
著者
-
Yamaya Tomoyuki
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
-
Yamaya T
Tohoku Univ. Sendai Jpn
-
Yamaya T
Institute For Agricultural And Biological Sciences. Okayama University
-
KOYAMA Hiroyuki
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University
-
Yamaya Tomoyuki
Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G
-
Ojima K
Tohoku Univ. Sendai Jpn
-
Ojima Kunihiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
-
Koyama H
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
-
Koyama Hiroyuki
Laboratory Of Plant Cell Technology Faculty Of Applied Biological Sciences Gifu University
-
Koyama Hiroyuki
Laboratory Of Plant Cell Technology Department Of Biotechnology Faculty Of Agriculture Gifu Universi
-
Koyama Hiroyuki
Laboratory Of Animal Physiology And Functional Anatomy Graduate School Of Agriculture Kyoto University
関連論文
- Cytosolic glutamine synthetase is present in the phloem sap of rice (Oryza sativa L.)(Plant Nutrition)
- Differential Distribution of Proteins Expressed in Companion Cells in the Sieve Element-Companion Cell Complex of Rice Plants
- DETECTION OF GREEN FLUORESCENCE PROTEIN AND β-GLUCURONIDASE PROTEIN IN THE PHLOEM SAP OF TRANSGENIC RICE PLANTS
- Multiple Polypeptides of Glutamine Synthetase Subunit in Rice Roots In Vivo and In Vitro(Biological Chemistry)
- Response of Glutamine Synthetase and Glutamate Synthase Isoforms to Nitrogen Sources in Rice Cell Cultures
- Immunochemical Analysis of Multiple Subunit Polypeptides of Glutamine Synthetase in Methionine Sulfoximine-Sensitive and Tolerant Tobacco Cell Cultures
- Organ and Cellular Localization of Asparagine Synthetase in Rice Plants
- Organ distribution of asparagine synthetase in rice plants
- Analyses of the transgenic rice plants expression antisense NADH-GOGAT RNA
- Transcriptomic profiling of major carbon and amino acid metabolism in the roots of Arabidopsis thaliana treated with various rhizotoxic ions(Plant Nutrition)
- Effect of Aluminum and Silicon in Solution on the Growth of Rice (Oryza sativa L.) Suspension Cells
- 9-17 INTERACTIONS BETWEEN ALIMINUM AND SILICON IN THE SUSPENSION CULTURE OF RICE (ORYZA SATIVA L.) CELLS
- Varietal Differences in the Growth of Rice Plants in Response to Aluminum and Silicon
- Aluminum-Induced Lipid Peroxidation and Lignin Deposition Are Associated with an Increase in H_2O_2 Generation in Wheat Seedlings (Plant Nutrition)
- Boron-Calcium Synergically Alleviates Aluminum Toxicity in Wheat Plants : Triticum aestivum L.(Plant Nutrition)
- Combined Effects of Mg and Ca Supply on Alleviation of Al Toxicity in Wheat Plants(Plant Nutrition)
- Effects of Aluminum and Boron Supply on Growth of Seedlings among 15 Cultivars of Wheat (Triticum aestivum L.) Grown in Bangladesh(Plant Nutrition)
- 9-11 Microscopic investigation on root apical cells of Al-stressed wheat seedlings under various concentrations of Ca and Mg
- Overexpressin of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil
- Over Expression of Mitochondrial Citrate Synthase Gene Improves the Growth of Carrot Cells in Al-Phosphate Medium
- Structure and Expression of the Mitochondrial Citrate Synthase Gene in Carrot Cells Utilizing Al-Phosphate
- Characteristics of Ammonium Uptake by Rice Cells in Suspension Culture
- ANALYSIS OF TRANSGENIC RICE EXPRESSING ANTISENSE RNA FOR CYTOSOLIC GLUTAMINE SYNTHETASE
- Over expression of mitochondrial citrate synthase in Arabidopsis thaliana alters citrate excretion from root
- Aluminum-Tolerance of Carrot (Daucus carota L.) Plants Regenerated from Selected Cell Cultures
- Gln49 and Ser174 Residues Play Critical Roles in Determining the Catalytic Efficiencies of Plant Glutamine Synthetase
- Biochemical Background and Compartmentalized Functions of Cytosolic Glutamine Synthetase for Active Ammonium Assimilation in Rice Roots
- Simple Identification of Transgenic Arabidopsis Plants Carrying a Single Copy of the Integrated Gene
- Alteration of Citrate Metabolism in Cluster Roots of White Lupin
- MOLECULAR CHARACTERIZATION OF CARBOHYDRATE METABOLISM OF ALUMINUM PHOSPHATE UTILIZING CELLS IN CARROT (Daucus carota L. )
- Organization and Structure of Ferredoxin - Dependent Clutamate Synthase Gene and Intracellular Localization of the Enzyme Protein in Rice Plants
- Analysis of the transgenic indica rice expressing NADH-dependent glutamate-synthase gene
- Increased Rubisco Content in Transgenic Rice Transformed with the 'Sense' rbcS Gene
- Expression of NADH-Dependent Glutamate Synthase in Response to the Supply of Nitrogen in Rice Cells in Suspension Culture
- A Supply of Nitrogen Causes Increase in the Level of NADH-Dependent Glutamate Synthase Protein and in the Activity of the Enzyme in Roots of Rice Seedlings : GROWTH AND DEVELOPMENT : PROTEINS, ENZYMES AND METABOLISM
- Molecular Basis on Nitrogen Utilization in Rice(Recent Topics of the Agricultunal Biological Science in Tohoku University)
- Mechanism of Nitrogen Remobilization in Rice(Frontiers in Rice Science -from Gene to Field-,The 100^ Anniversary of Tohoku University, International Symposium)
- Tissue and Cellular Localization of NADH-Dependent Glutamate Synthase Protein in Leaves of Spinach
- ACT Domain Repeat Protein 7, ACR7, Interacts with a Chaperone HSP18.0-CII in Rice Nuclei
- Interaction of N-Acetylglutamate Kinase with a PII-Like Protein in Rice
- A Ca^-Dependent Protein Kinase that Endows Rice Plants with Cold- and Salt-Stress Tolerance Functions in Vascular Bundles
- Compartmentation of asparagine synthetase in rice roots
- Investigation of cis element that regulates tissue-specific expression of gene for rice NADH-GOGAT
- LOCALIZATION OF STRESS-INDUCIBLE OsCDPK7 SIGNALING PATHWAY IN WILD-TYPE AND OsCDPK7-OVEREXPRESSING RICE PLANTS
- Cellular localization of asparagine synthetase in rice plants
- Cellular and subcellular localization of NADH-dependent or Fd-dependent glutamate synthase protein in the stele of rice roots
- REGULATION OF THE EXPRESSION OF NADH-DEPENDENT GLUTAMATE SYNTHASE (NADH-GOGAT) GENE BY OKADAIC ACID IN RICE
- Inducible Accumulation of mRNA for NADH-Dependent Glutamate Synthase in Rice Roots in Response to Ammonium Ions
- Tissue specific and nitrogen inducible expression of rice NADH-GOGAT gene promoter in transgenic rice plants
- ANALYSIS OF NADH DEPENDENT GLUTAMATE SYNTHASE PROMOTER IN TRANSGENIC RICE PLANTS
- CHARACTERIZATION OF RICE NADH-DEPENDENT GLUTAMATE SYNTHASE cDNA
- STRUCTURAL CHARACTERIZATION OF RICE NADH-DEPENDENT GLUTAMATE SYNTHASE GENE
- MOLECULAR CLONING AND STRUCTURAL CHAR-ACTERIZATION OF RICE NADH-DEPENDENT GLU-TAMATE SYNTHASE GENE
- Aluminum Tolerance of Arabidopsis thaliana under Hydroponic and Soil Culture Conditions
- Organic Acid Metabolism in Aluminum-Phosphate Utilizing Cells of Carrot : Daucus carota L.
- A Simple Hydroponic Culture Method for the Development of a Highly Viable Root System in Arabidopsis thaliana
- CHARACTERIZATION OF AN ACID-SOIL-ADAPTED ECOTYPE OF Arabidopsis thaliana
- ALTERATION OF CARBHYDRATE METABOLISM IN ALUMINUM PHOSPHATE UTILIZING CARROT (Daucus carota L.) CELLS
- Direct Observation of Root-Elongation of Arabidopsis thaliana Seedlings Grown in Hydroponic Culture
- Changes in the tissue-localization of NADH-dependent glutamate synthase protein following the addition of NH_4^+ in the root-tips of rice seedlings
- RESPONSE OF NADH-DEPENDENT GLUTAMATESYNTHASE (NADE-GOGAT) TO AMMONIUM ION IN ROOTS OF RICE SEEDLINGS
- Transgenic rice plants whose expression of NADH-GOGAT is modulated by anti-sense RNA
- Factors Affecting Aluminum Tolerance of Cells in Carrot (Daucus carota L.) Suspension Cells
- CHARACTERIZATION OF TWO TOBACCO CELL LINES SELECTED TO GROW IN THE PRESENCE OF EITHER IONIC AI OR INSOLUBLE Al-PHOSPHATE
- P11-15 Transcriptomic characterization of Arabidopsis roots exposed to various rhizotoxic treatments giving 50% of growth inhibition
- Histochemical studies on the expression of two glutamate synthase proteins and mRNAs in rice roots
- Characterization of Citrate Transport through the Plasma Membrane in a Carrot Mutant Cell Line with Enhanced Citrate Excretion
- Effects of Aluminum and pH on Root Growth and Cell Viability in Arabidopsis thaliana Strain Landsberg in Hydroponic Culture : ENVIRONMENTAL AND STRESS RESPONSES
- Characteristics of Aluminum-Phosphate-Adapted Carrot Cells: Uptake and Utilization of the Phosphate
- Utilization of Anhydrous Aluminum Phosphate as a Sole Source of Phosphorous by a Selected Carrot Cell Line
- ANALYSIS OF PHENYLTHIOCARBAMYL-AMINO ACIDS AT PICO-MOLE LEVEL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND APPLICATION TO PLANT MATERIALS
- N-Salicylidene-2-aminopyridine as a Photochromic Guest for Deoxycholic Acid Crystal Lattice Cavities
- QTL Analysis of Al Tolerance in Recombinant Inbred Lines of Arabidopsis thaliana
- 11-10 Comparison of global gene expression in Arabidopsis roots facing different levels of rhizotoxic ion stress : Comparison of gene expression in Arabidopsis roots
- Roles of One-carbon Metabolism in Preimplantation Period : Effects on Short-term Development and Long-term Programming