Characteristics of Ammonium Uptake by Rice Cells in Suspension Culture
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概要
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As a model system that dose not involve long distance transport, cell cultures of rice (Oryza sativa L. cv Sasanishiki) were used to investigate the characteristics of NH_4^+ uptake. The cells, which had been subcultured or 8 d in normal R2 medium containing 5 mM NH_4^* and 40 mM NO_3^-, were pre-cultured for another 2.5 d without nitrogen and then used as the materials. In some experiments, the cells were further treated with 10 μM of methionine sulfoximine (MSX) for 3 h in the medium lacking nitrogen to inhibit the endogenous glutamine synthetase activity. Uptake of NH_4^+ was determined by measuring the consumption of NH_4^+ from 25 mL of an uptake medium containing 25 mM 2-morpholinoethanesulfonic acid-NaOH (pH 5.6), 100μM CaCl_2, 100 μM NH_4Cl, and 0.5 g fresh weight cells. When the uptake was followed as a function of time, the cells treated with MSX rapidly absorbed NH_4^+ during the first 20 min and then the absorption leveled off. The cells without MSX treatment absorbed the ion at the same rate for 40 min and then the absorption leveled off. The uptake rate during the first 20 min was calculated to be 90 nmol NH_4^+ per min per g fresh weight. Intracellular concentrations of NH_4^+ were at least several times higher than those of the medium regardless of MSX treatment throughout the period of incubation. A biphasic saturation curve for the uptake of NH_4^+, with an apparent K_m of 240 M and 1.7 mM, was obtained when the cells were incubated in the medium containing 0 to 500 M of the ion. The rice cells showed a pH optimum of about 7.0 for the uptake of NH_4^+. The uptake was strongly inhibited by a proton conductor and an inhibitor of H^+-ATPase. The results of these kinetic studies suggest that NH_4^+ is transported via a carrier system across the cell membrane, but not via a simple diffusion mechanism, in rice cell cultures.
- 社団法人日本土壌肥料学会の論文
著者
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Mae Tadahiko
Laboratory Of Plant Nutrition And Function Graduate School Of Agricultural Science Tohoku University
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Mae Tadahiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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Karasawa Toshihiko
Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological
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Hayakawa Toshihiko
Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological
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Ojima Kunihiko
Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological
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Yamaya Tornoyuki
Laboratory of Plant Nutrition and Laboratory of Cell Biochemistry, Department of Applied Biological
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Ojima Kunihiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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Hayakawa Toshihiko
Laboratory Of Plant Cell Biochemistry Department Of Applied Plant Science Division Of Life Science G
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Hayakawa Toshihiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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Karasawa Toshihiko
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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Yamaya Tornoyuki
Laboratory Of Plant Nutrition And Laboratory Of Cell Biochemistry Department Of Applied Biological C
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