RNA Extraction from Various Recalcitrant Plant Tissues with a Cethyltrimethylammonium Bromide-Containing Buffer Followed by an Acid Guanidium Thiocyanate-Phenol-Chloroform Treatment

元データ 2008-07-23

概要

High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds of Lotus japonicus and rice, sweet potato tuber, banana fruit). This protocol greatly reduced the time required for RNA extraction.

著者

牧野 周 東北大院農
牧野 周 東北大院・農
牧野 周 東北大学農学部
Mae Tadahiko Laboratory Of Plant Nutrition And Function Graduate School Of Agricultural Science Tohoku University
Makino Amane Laboratory Of Plant Nutrition And Function Graduate School Of Agricultural Science Tohoku University
SUZUKI Yuji Laboratory of Plant Environmental Responses, Graduate School of Agricultural Science, Tohoku Univers
Mae Tadahiko Dept.appl.biol.chem. Fac.agric. Tohoku Univ.
Suzuki Yasuhiro Graduate School Of Agricultural Science Tohoku University

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