Dehydroepiandrosterone Sulfateのin Vitro代謝(第1報)ヒトおよびサル妊娠末期胎盤における代謝

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The in vitro metabolism of dehydroepiandrosterone sulfate (DHAS) was investigated in placentas at term from human subjects and monkeys (Macaca fascicularis). In each case DHAS was easily hydrolyzed to unconjugated metabolites and dehydroepiandrosterone (DHA), androst-4-ene-3,17-dione (Δ^4-A-dione), androst-5-ene-3β, 17β-diol (Δ^5-A-diol), testosterone (T), estrone (E_1) and estradiol-17β (E_2) were identified as metabolites by two dimensional thin layer chromatography and reverse isotope dilution technique. DHAS was rapidly converted to E_1 and E_2 which were already main metabolites after incubation for 20 min with human placentas in the presence of NADPH as coenzyme. The conversion of the substrate to E_1 and E_2 after incubation for 2 hr was in the range of 45 to 67% and 7 to 16%, respectively After the incubation with monkey placentas, DHA was a main metabolite and the activity to convert the substrate to E_1 and E_2 was considerably lower than that observed in human placental preparations.
公益社団法人日本薬学会の論文
1980-11-25