高速液体クロマトグラフ法による輸液中の水溶性ビタミンの定量(第2報)ピリジン-4-アルデヒドを用いたリン酸ピリドキサールの分析

概要

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A high performance liquid chromatographic method is described for the determination of pyridoxal-5'-phosphate (PLP) in injections. PLP in injections exists as an adduct of sodium bisulfite, and is not easily separated on the reverse-phase C_<18> column as a single peak. The present method is based on the conversion of the adduct to PLP by treatment with excess pyridine-4-aldehyde. The recovery of PLP from various infusion fluids added with PLP was 97.6-100.5%. The recovery of PLP in the presence of its photodecomposition products was 98.1-102.8%. The method was not influenced by the constituents of infusion fluids and PLP photodecomposition products. The method provided a simple and rapid procedure for the evaluation of the quality and stability of PLP preparations under clinical conditions.
公益社団法人日本薬学会の論文
1985-11-25