Studies on Preconcentration Methods for Detecting the Mutagenicity of Organics in Drinking Water

概要

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As a pretreatment in mutagenicity assays of non-volatile organic micropollutants in drinking water, the applicability of three methods of concentration (solvent extraction, XAD-2 column extraction and lyophilization) was investigated in conjunction with Ames Salmonella/microsome assays. Raw and tap water samples and ozonated and chlorinated humic samples used were assayed with strains TA 98,TA 100 and TA 104. When lyophilization was employed, the detection of the mutagenicity of organic micropollutants in tap water required at least 10000-fold concentration, and as a solvent of mutagenic materials in tap water, DMSO was more effective than distilled water. Of the three preconcentration methods, the acidic extracts in the ethyl ether extraction and the dichloromethane fractions in the XAD-2 column extraction were found to recover selectively mutagenic and non-volatile organic materials from all of raw water, tap water and chlorinated humic samples. In particular, the XAD-2-dichloromethane fraction was considered to recover quantitatively mutagenic products from chlorinated humic samples. These findings indicate that XAD-2 column extraction may be the most suitable preconcentration procedure for organic micropollutants in water.
公益社団法人日本薬学会の論文
1987-10-31