有害性金属の衛生化学的研究(第4報)カドミウムの生体内への吸収機構について

元データ 1976-02-29 公益社団法人日本薬学会

概要

The behavior of cadmium absorbed through intestine into living organisms was examined in detail. Metabolism of cadmium such as the interaction in intestinal mucosal precipitate fraction, dynamic behaivior of absorption through intestine to blood, and binding form in serum, liver, and kidney was investigated. Rats administered orally with 1 μCi of ^<109>CdCl_2 as 1 mg/ml solution were sacrificed after 20 min or 2 hr. The intestine, liver, kidney, and blood were homogenised in Tris-HCl buffer solution, and separated into subcellular fractions by centrifugation. The supernatant of each organ was fractionated by gel filtration over Sephadex G-75. Radioactivity of ^<109>Cd of the preparation was determined. Most of orally dosed cadmium was bound charactaristically in the intestinal mucosal precipitate fraction, because cadmium bound to the mucosal precipitate could not be isolated by dialysis with Tris-HCl buffer solution or 10^<-4>M ethylenediaminetetraacetic acid (EDTA) solution but was isolated by 10^<-2>M EDTA solution. Cadmium was absorbed in the form of free cadmium, which was identified in the liver, kidney, and serum by gel filtration. Cadmium in the supernatant of these organs was present as Fr-I (high molecular weight), Fr-II (metallothionein), and Fr-III (free cadmium). Cadmium in Fr-I decreased with passage of time in all organs, but that in Fr-II increased 2 hr after administration, especially in the liver. There was free cadmium in all the organs 20 min after the administration but not after 2 hr.

著者

安藤 正典 国立衛生試験所
佐谷戸 安好 国立衛生試験所
佐谷戸 安好 摂南大学・薬学部
佐谷戸 安好 国立衛生試験所環境衛生化学部
外村 正治 国立衛生試験所

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