ヘマトポルフィリン等のUVA増感による過酸化脂質生成と赤血球の溶血反応との関連

概要

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To examine biological effects of UVA, we investigated effects of UVA-sensitization with hematoporphyrin (HP) or riboflavin (RF) on the hemolysis and lipid hydroperoxide levels of rabbit erythrocytes. We irradiated UVA (365 nm) at r.t. for certain time, and after the end of irradiation measured hemolysis keeping erythrocytes at r.t. or at 0℃. As the result, though erythrocytes hemolyzed almost 100% at r.t., hemolysis was almost 0% at 0℃. It is evident that HP was a much stronger sensitizer than RF. Moreover, we measured contents of hydroperoxide of phosphatidylcholine (PC) and phosphatidylethanolamine (PE), most of which constructed lipid cellular membrane, after or before UVA irradiation by the chemiluminescence-HPLC method. As the result, before hemolysis, hydroperoxide levels per lipid significantly increased after the end of irradiation, compared with control (1.75→4.57 nmol/mg PC. 6.13→12.9 nmol/mg PE, respectively). These results suggest that celluar membrane lipid hydroperoxide which was formed with singlet oxygen produced by UVA sensitization, might be concerned with hemolysis.
社団法人日本薬学会の論文
1994-12-31