Blue Fluorescence Generated during Lipid Oxidation of Rat Liver Microsomes Cannot Be Derived from Malonaldehyde but Can Be from Other Aldehyde Species
スポンサーリンク
概要
- 論文の詳細を見る
Generation of blue fluorescence together with phospholipid hydroperoxides and aldehyde species in rat liver microsomes during oxidation with FeCl_2-ADP-ascorbic acid was monitored, and the kind of lipid oxidation products participating in the formation of blue fluorescence was investigated. Contents of phospholipid hydroperoxides were incresed in an early stage of oxidation, and were decreased in an advanced stage of oxidation. Contents of components that liberated malonaldehyde, 4-hydroxyalkenals and other unsaturated aldehydes under the acidic assay conditions were increased in the advanced stage of oxidation. Water-soluble blue fluorescence with a maximum at 440-450nm determined after separation through gel filtration accumulated in the advanced stage of oxidation, and was characterized as resistant to borohydride treatment and to be little dependent on pH values of the solvent. Wavelength of the maximum fluorescence and characteristics of the fluorescence were similar to those of fluorescence with maxima at 440-450nm formed by reaction of unoxidized microsomes, bovine serum albumin or methylamine with alkenals, and different from those of fluorescence with maxima at above 460nm obtained by the reaction with a mixture containing malonaldehyde. Hence, blue fluorescence accumulated in oxidized microsomes cannot be derived from free malonaldehyde but can be from other aldehyde species including alkenals.
- 公益社団法人日本薬学会の論文
- 1998-04-15
著者
-
KIKUGAWA Kiyomi
School of Pharmacy, Tokyo University of Pharmacy and Life Science
-
Kikugawa Kiyomi
School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Kikugawa Kiyomi
School Of Pharmacy Tokyo University Of Pharmacy And Life Science (formerly Tokyo College Of Pharmacy
-
INOUE Tadamichi
School of Pharmacy, Tokyo University of Pharmacy and Life Science (Formerly Tokyo College of Pharmac
-
Inoue Tadamichi
School Of Pharmacy Tokyo University Of Pharmacy And Life Science (formerly Tokyo College Of Pharmacy
関連論文
- Characterization of Lactoferrin-Binding Proteins of Human Macrophage Membrane : Multiple Species of Lactoferrin-Binding Proteins with Polylactosamine-Binding Ability
- Recognition of Poly-N-acetyllactosaminyl Saccharide Chains on Iron-Oxidized Erythrocytes by Human Monocytic Leukemia Cell Line THP-1 Differentiated into Macrophages
- Binding Characteristics of Human Lactoferrin to the Human Monocytic Leukemia Cell Line THP-1 Differentiated into macrophages
- Effect of Supplementation of n-3 Polyunsaturated Fatty Acids on Oxidative Stress-Induced DNA Damage of Rat Hepatocytes(Biochemistry/Molecular Biology)
- Effect of Supplementation of a Reductone in Coffee, Hydroxyhydroquinone, on Lipid Peroxidation and DNA Damage of Rat Organs
- Inhibition of Iron-Induced Oxidative Damage of Erythrocyte Membranes and Low Density Lipoprotein by a Mailard Product, 4-Hydroxy-2(or 5)-ethyl-5(or 2)-methyl-3(2H-furanone (HEMF)
- Effect of Feeding Plant Polyphenolics on Fatty Acid Composition, Vitamin E Level and Degree of Lipid Peroxidation in Rat Organs
- Participation of the Arachidonic Acid Cascade Pathway in Macrophage Binding / Uptake of Oxidized Low Density Lipoprotein
- Inhibition of the Activity of Mouse Macrophage Scavenger Receptors by Antioxidants
- Cholesterol Synthesis in Mice Is Suppressed but Lipofuscin Formation Is Not Affected by Long-Term Feeding of n-3 Fatty Acid-Enriched Oils Compared with Lard and n-6 Fatty Acid-Enriched Oils
- Inhibition of Protein Phosphorylation in Macrophages by Antioxidants
- Water-soluble Antioxidants Inhibit Macrophage Recognition of Oxidized Erythrocytes
- Enhanced Adhesion of Oxidized Mouse Polymorphonuclear Leukocytes to Macrophages by a Cell-Surface Sugar-Dependent Mechanism
- Formation of Hydroxyfuranone and Hydroxypyranone Derivatives with DNA-Breaking Activity in the Maillard Reaction of Glucose and Albumin under Physiological Conditions
- Strategy in a Living Body to Protect against Oxidative Stress-Induced Damage
- Blue Fluorescence Generated during Lipid Oxidation of Rat Liver Microsomes Cannot Be Derived from Malonaldehyde but Can Be from Other Aldehyde Species
- Mutagenicity of Cooked Hamburger is Reduced by Addition of Ascorbate and Erythorbate to Ground Beef
- Identification of the Cleavage Sites of Oxidized Protein That Are Susceptible to Oxidized Protein Hydrolase (OPH) in the Primary and Tertiary Structures of the Protein
- Is Nitric Oxide (NO) an Antioxidant or a Prooxidant for Lipid Peroxidation?
- Suppression of Free Radical-Induced DNA Strand Breaks by Linoleic Acid and Low Density Lipoprotein in Vitro(Toxicology)
- Presence of Oxidized Protein Hydrolase in Human Cell Lines, Rat Tissues, and Human/Rat Plasma^1
- Purification and Characterization of a Serine Protease in Erythrocyte Cytosol That Is Adherent to Oxidized Membraanes and Preferentially Degrades Proteins Modified by Oxidation and Glycation
- Macrophage Binding and the Uptake of Oxidized Low Density Lipoprotein Are Regulated by Intracellular Protein Phosphorylation
- Enzymatic Removal of Oxidized Protein Aggregates from Erythrocyte Membranes
- Is Nitrotyrosine Generated in Human Erythrocytes in Circulation?
- Increased Band 3 Protein Aggregation and Anti-band 3 Binding of Erythrocyte Membranes on Treatment with Sesamol
- Binding of Anti-Band 3 Autoantibody to Sialylated Poly-N-Acetyllactosaminyl sugan Chains of Band 3 Glycoprotein on Eviednce for Anti-Band 3 Autoantibody Binding to the Sugar Chains of Oxidized and Senescent Erythrocytes
- Are Water-Soluble Natural Antioxidants Synergistic in Combination with α-Tocopherol?
- Vitamin E at Physiological Levels Enhances Mouse Macrophages to Bind and Incorporate Oxidized Low Density Lipoprotein
- Mouse Liver Microsomal Lipid Peroxidation and Antioxidant Effect of α-Tocopherol at the Low Oxygen Concentration
- Effects of Vitamin E-Deficiency and/or Nitrogen Dioxide Inhalation on Allergen-Sensitized Type IV and Type I Allergy Responses of Mice
- Low Inhibitory Activities of Food Phenolics Against Binding of Estradiol to Human Estrogen Receptor α
- Generation of Hydrogen Peroxide from Hydroxyhydroquinone and Its Inhibition by Superoxide Dismutase
- Increased Urinary Hydrogen Peroxide Levels Caused by Coffee Drinking
- Effect of Vitamin E on Contact Sensitization Responses Induced by 2, 4-Dinitrochlorobenzene in Mice