Characterization of Human p33/41 (Annexin IV), a Ca^<2+> Dependent Carbohydrate-Binding Protein with Monoclonal Anti-annexin IV Antibodies, AS11 and AS17
スポンサーリンク
概要
- 論文の詳細を見る
p33/41 (annexin IV) is a member of the family of Ca^<2+>-dependent phospholipid binding proteins known as annexins. We previously described that bovine kidney p33/41 (annexin IV) has Ca^<2+>-dependent carbohydrate binding acrivity. In this study, we purified human p33/41 (annexin IV) from the HT29,human colon adenocarcianoma cell line, as well as the bovine kidney annexin by affinity chromatography. Then, we prepared recombinant human p33/41 (annexin IV) expressed in Escherichia coli. The apparent size and the Ca^<2+>-dependent carbohydrate binding properties of purified recombinant p33/41 (annexin IV) were indistinguishable from those of the bovine kidney protein. We also performed inhibition assays of carbohydrate binding and of phosphatidylserine/phosphatidylcholine liposome binding of recombinant p33/41 (annexin IV) with anti-p33/41 monoclonal antibodies (AS11 and AS17). We determined the epitopes recognized by the monoclonal antibodies by Western blot analysis using deleted-recombinant p33/41 (annexin IV). The monoclonal antibodies recognized domain 1 and/or 2 of p33/41 (annexin IV). The results of the inhibition assays and the determination of the epitope showed that a carbohydrate binding site is located at domains 3 and 4 of p33/41 (annexin IV) and on the cell surface.
- 公益社団法人日本薬学会の論文
- 1997-03-15
著者
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Matsumoto I
お茶の水女子大学
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Matsumoto Isamu
The Graduate School Of Humanities And Sciences Ochanomizu University
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KOJIMA Kyoko
Department of Internal Medicine, Teikyo University School of Medicine
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Kojima K
Kyushu Univ. Fukuoka
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Kojima Kyoko
Department Of Chemistry Faculty Of Science
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MATSUMOTO Isamu
Department of Obstetrics and Gynecology, Faculty of Medicine, Kagoshima University
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SATOH Ayano
The Graduate School of Humanities and Sciences, Ochanomizu University
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SATOH Ayano
Department of Chemistry, Faculty of Science, Ochanomizu University
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TAKAYAMA Eiji
National Defense medical College
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OGAWA Haruko
Department of Chemistry, Faculty of Science, Ochanomizu University
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KATSURA Yoshimoto
Department of Molecular Pathology & Immunology, Chest Disease Research Institute, Kyoto University
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KINA Tatsuo
Department of Molecular Pathology & Immunology, Chest Disease Research Institute, Kyoto University
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Ogawa Haruko
Department Of Chemistry Faculty Of Science Ochanomizu University
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Ogawa Haruko
Department Of Advanced Biosciences Graduate School Of Humanities And Sciences
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Ogawa Haruko
Department Of Advanced Biosciences Graduate School Of Humanities And Sciences Ochanomizu University
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Kojima K
Graduate School Of Pharmaceutical Sciences Nagoya City University
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Matsumoto I
Ochanomizu Univ. Tokyo
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Matsumoto Isamu
Matsumoto Institute Of Life Science
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Matsumoto Isamu
Division Of Human Genetics Medical Research Institute Kanazawa Medical University
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Matsumoto Isamu
Department Of Chemistry Faculty Of Science Ochanomizu University
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Matsumoto Isamu
Graduate School Of Humanities And Sciences Ochanomizu University
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Kina T
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Kina Tatsuo
Department Of Bacteriology And Serology Chest Disease Research Institute Kyoto University
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Katsura Y
Department Of Molecular Pathology & Immunology Chest Disease Research Institute Kyoto University
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Katsura Yoshimoto
Department Of Immunology Chest Disease Research Institute Kyoto University
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Satoh A
The Graduate School Of Humanities And Sciences Ochanomizu University
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MATSUMOTO ISAMU
Department of Biochemistry, Kurume University School of Medicine
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KATSURA Yoshimoto
Department of Bacteriology and Serology, Chest Disease Research Institute, Kyoto University
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