Differentiation and Maturation of Functional T Lymphocyte Subsets in the Thymus I. Effect of Irradiation of the Thymus

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概要

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• この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。Effect of the irradiation of the thymus on the development of T cells was investigated. C57BL/6 (H-2^b, Thy-1.2) mice were irradiated without shielding or with shielding over the thymus or over the right leg and tail, and they were transferred with bone marrow cells from B10. Thy-1.1 (H-2^b, Thy-1.1) mice. After the reconstitution, the proportion of donor-type Thy-1.1 bearing cells were analysed by flow cytofluorometry. In the whole-body irradiated mice thymus cells were nearly completely replaced by donor derived cells within 21 days. In thymus-shielded mice and right-leg-and-tail-shielded mice, about half of the thymus cells were donor-type cells at 28 days after the reconstitution. The development of donor-derived cytotoxic T cell precursors (CTLp) and helper T cells (Th) in the thymus of these recipient mice was investigated. In order to increase the sensitivity of detection, concanavalin A-mediated polyclonal assay system was used for both CTLp and Th, and the PNA^- cell fraction of thymocytes was used as a source of functional cells. In whole-body irradiated mice and in right-leg-and-tail-shielded and irradiated mice, CTLp developed before Th did, whereas the reverse was true in thymus-shielded and irradiated mice. These results strongly suggested that the capacity of the thymus to allow the proliferation of donor type Thy-1 bearing cells and the development of CTLp in the thymus was independent of whether or not this organ was irradiated. On the other hand, the development of functional Th was obviously retarded by the irradiation on the thymus, suggesting that the radiosensitive thymic element plays a critical role for the generation of Th.

• 京都大学の論文
• 1986-08-31

著者

• Kina T

  Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University

• Kina Tatsuo

  Department Of Bacteriology And Serology Chest Disease Research Institute Kyoto University

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