The Average Unwinding Angle of DNA Duplex Produced by the Binding of Chromomycin A_3

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概要

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• The effect of chromomycin A_3 binding on the geomtery of DNA deplex (plasmid pBR322) has been examined using topoisomerase I relaxation followed by gel electrophoresis. To determine the equilibrium constant of this drug-DNA binding-dissociation reaction in the same concentration range (ca. 10^<-5>M) in the same buffer as those for the topoisomerase reaction (at 37℃), fluorescence measurements were made of the same plasmid-drug system, followed by a Scatchard plot and an analysis using McGhee-von Hippel's exclusion site model. The binding constant has been fouond to be 3.8×10^5M^<-1> in the particular buffer (buffer-T) at 37℃, and the number of base pairs involved in the site of one chromomycin molecule on the duplex has been found to be 5. It has been concluded that one chromomycin molecule, bound to the duplex, unwinds it by 11.8±1.1 degress. In addition, the enthalpy of binding was determined to be 31.81 kJ/mole using a titration calorimeter with a more concentrated (6.2 mM) solution.

• 公益社団法人日本薬学会の論文
• 1998-11-15

著者

• Kojima K

  Kyushu Univ. Fukuoka

• MAEDA Yoshiko

  Showa Pharmaceutical University

• Maeda Y

  Showa Pharmaceutical University

• Utsuno K

  High-tech Research Center College Of Science And Engineering Iwaki Meisei University

• TSUBOI Masamichi

  High-Tech Research Center, College of science and Engineering, Iwaki Meisei University

• MAEDA Yoshimi

  Department of Fundamental Science,Iwaki Meisei University

• Maeda Yoshimi

  Department Of Fundamental Science Iwaki Meisei University

• UTSUNO Kuniharu

  Department of Fundamental Science, Iwaki-Meisei University

• TSUBOI Masamichi

  Department of Fundamental Science, Iwaki-Meisei University

• KOJIMA Katsuko

  Department of Fundamental Science, Iwaki-Meisei University

• Kojima Katsuko

  Department Of Fundamental Science Iwaki-meisei University

• Tsuboi M

  High-tech Research Center College Of Science And Engineering Iwaki Meisei University

• Tsuboi Masamichi

  Department Of Chemistry Faculty Of Science Tokyo University

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