Visualization of Complexes of Hoechst 33258 and DNA Duplexes in Solution by Atomic Force Microscopy
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概要
- 論文の詳細を見る
Tertiary structure changes in DNA duplexes, induced by Hoechst 33258 binding, have been examined by the use of atomic force microscopy. Besides minor groove binding, which is an established mode of binding for this drug, Hoechst 33258 has now been found to show another binding mode, which causes an unwinding of the duplex. When the drug concentration is as high as 0.5μg/ml, the Hoechst 33258 molecule seems to function as a clamp for two DNA chains and forms a condensate. The condensate was found to have a toroidal shape. By surveying more than 100 microscopic images of such condensates formed in 1μg/ml drug solution, a mechanism of toroidal condensate formation has been proposed.
- 公益社団法人日本薬学会の論文
- 2002-02-01
著者
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Utsuno K
High-tech Research Center College Of Science And Engineering Iwaki Meisei University
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Iwamoto T
High-tech Research Center College Of Science And Engineering Iwaki Meisei University
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UTSUNO Kuniharu
High-Tech Research Center, College of Science and Engineering, Iwaki Meisei University
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TSUBOI Masamichi
High-Tech Research Center, College of science and Engineering, Iwaki Meisei University
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KATSUMATA Shunji
High-Tech Research Center, College of Science and Engineering, Iwaki Meisei University
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IWAMOTO Toschitake
High-Tech Research Center, College of Science and Engineering, Iwaki Meisei University
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UTSUNO Kuniharu
Department of Fundamental Science, Iwaki-Meisei University
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Katsumata S
High-tech Research Center College Of Science And Engineering Iwaki Meisei University
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Tsuboi M
High-tech Research Center College Of Science And Engineering Iwaki Meisei University
関連論文
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