Differentiation and maturation of functional T lymphocyte subsets in the thymus. II. Generation of T cell specificities and functions from a single stem cell.
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この論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。Functional differentiation of T cells in the thymus from a single stem cell was investigated by transferring bone marrow cells dirrectly into the thymus (i.t.) of irradiated and reconstituted mice, and assaying the immunologic function of donor drived T cells. To assure the clonality of donor derived T cells, bone marrow cells from two mutually indentifiable strains were 1 : 1 mixed and the limited number of the mixture was transferred i.t. into the recipient mice. As the donor of bone marrow cells, C57BL/6 (H-2^b, Thy-1.2,Lyt-2.2) and B6. Lyt-2.1 (H-2^b, Thy-1.2,Lyt-2.1) mice were used, and B10. Thy-1.1 (H-2^b, Thy-1.1,Lyt-2.2) mice were used as the recipients. Recipient mice were either thymus-shielded and irradiated or whole-body irradiated and reconstituted with 10^7 syngeneic bone marrow cells. The recipient's thymus which contained Thy-1.2^+ cells on day 28 were regarded as positive for donor derived cells, and among these positive thymuses those which contained either Lyt-2.1^+ cells or Lyt-2.2^+ cells were regarded as seeded by a single stem cell (single positive thymus cells). Such single positive thymus cells were treated with anti-Thy-1.1 plus complement to remove recipient-type T cells and the recovered cells were assayed for proliferative response and cytotoxic response in mixed lymphocyte culture against allogeneic lymphocytes. Cells were also assayed for concanavalin A-mediated polyclonal helper T cell (Th) and cytotoxic T cell (CTL) functions. Results indicated that proliferative response to alloantigens was observed only in thymus-shielded recipients, whereas CTL to alloantigens was generated in both thymus-shielded recipients and whole-body irradiated recipients. No general rule of preference for any allogeneic cells was found. Both Th and CTL activities, detectable by polyclonal assays, were generated from a single stem cell in thymus-shielded recipients. However, only CTL but not Th was generated in whole-body irradiated recipients. Moreover, a clone of T cells which responded with CTL to a given allogeneic cells did not necessarily respond with a marked proliferation to the same stimulator cells.
- 京都大学の論文
- 1987-08-31
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