Leguminous wood has a unique vacuolar lectin in bark : Lectin-carbohydrate interaction modulates solubility-insolubility transition of vacuolar glycoproteins

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概要

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• Mannose/glucose-specific sophoragrin, a Sophora japonica bark lectin, shows unique multimerization and dissociation accompanied by transitions between insolubility and solubility, which are modulated by both a specific sugar and Ca^<2+> concentration. Sophoragrin is composed of four subunits, a-1, a-2, b-1, and b-2, and all except for b-2 are reported to be glycosylated. Biotinylated sophoragrin bound sugar-specifically to subunit 'b' but not to subunit 'a' on the membrane. Sophoragrin was labeled by only one of the photoaffinity-probes derived from sophoragrin-glycans, and the labeling was inhibited by a specific sugar. In contrast, another probe-mixture, which contains major oligosaccharide of sophoragrin (Man_3Xyl_1Fuc_1GlcNAc_2), did not label sophoragrin. These results reveal that the multimerization of sophoragrin is caused by a particular signal oligosaccharide on subunit 'b'. It was shown that sophoragrin can form a soluble complex with other endogenous glycoproteins, and may play a specific role through its transition between soluble and insoluble forms.

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著者

• UEDA Haruko

  Department of Botany, Graduate School of Science, Kyoto University

• OGAWA Haruko

  Department of Chemistry, Faculty of Science, Ochanomizu University

• Hatanaka Yasumaru

  Institute Of Natural Medicine Toyama Medical And Pharmaceutical University

• Ogawa Haruko

  Department Of Chemistry Faculty Of Science Ochanomizu University

• Ogawa Haruko

  Department Of Advanced Biosciences Graduate School Of Humanities And Sciences

• Ogawa Haruko

  Department Of Advanced Biosciences Graduate School Of Humanities And Sciences Ochanomizu University

• Ueda Haruko

  Department Of Advanced Biosciences Graduate School Of Humanities And Sciences Ochanomizu University:

• Ueda Haruko

  Department Of Advanced Biosciences Graduate School Of Humanities And Sciences

• Hatanaka Yasumaru

  Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

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