Screening of Natural Compounds for Inhibitory Activity on Colon Cancer Cell Migration

概要

論文の詳細を見る
We examined the effects of 75 kinds of natural compounds, such as alkaloids, phenylpropanoids, flavonoids, steroids and terpenoids on the in vitro migration and proliferation of colon 26-L5 cells, in comparison with anticancer drugs used for chemotherapy. Twenty-three of the 75 compounds inhibitory markedly tumor cell migration. Among the 23 compounds, evodiamine showed the most potent and selective inhibitory activity on tumor cell migration with an IC_<50> value of 1.25μg/ml, which was about 20 times lower than that for tumor cell proliferation. The migratory inhibition reached acout 70% at 10μg/ml of evodiamine. On the other hand, most of anticancer drugs tested, except for paclitaxel, had little effect on tumor cell migration at the concentrations strongly inhibiting tumor cell proliferation. Paciraxel suppressed tumor cell migration in a concentrations strongly inhibiting tumor cell proliferation. Partitaxel suppressed tumor cell migration in a concentration-dependent manner and achieved about 70% inhibition at 10μg/ml with a marginal effect on cell proliferation. These results suggest that evodiamine and paciltaxel may be regarded as leading compounds for anti-metastatic agents acting through the inhibition of tumor cell migration without cytotoxicity.
公益社団法人日本薬学会の論文
2001-06-01