Component Determination of Total Saponins in Bupleurum Root by High-performance Liquid Chromatography

概要

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Considering not only free saponins (saikosaponin a and saikosaponin d), but also their acylsaponins, the determination for the total amount of saponins in Bupleurum Root was studied. This paper especially describes the optimization of the suitable analytical conditions. A diluted sodium hydroxide test solution (TS) was added to the extract of the sample to saponify acylsaponis. The mixture was neutralized and treated using an octadecylsilanized silica gel (ODS) cartridge column to furnish a sample solution, and analyzed with a HPLC technique using an ODS column. The total amount of saponins, which was defined as the sum amount of saikosaponins a and saikosaponin d, was from 0.33 to 1.49% (average 0.78%). The amount of saikosaponin a increased 1.5 times and that of saikosaponin d did 1.6 times as the result of saponification. The method mentioned above is assumed to be time saving because plural samples can be handled in parallel.
日本生薬学会の論文
2004-08-20