Charasteristics of Anti-testosterone Antisera Produced by Bovine Serum Albumin Conjugates of 15α-and 15β-Carboxymethyltestosterone : Use of [^<125>I]iodinated Tracers
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概要
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Each testosterone-[^<125>I]iodinated histamine derivative where[^<125>I)iodinated histamines were linked to respective 15α- and 15β-carboxymethyltestosterone (15α-and 15β-CMT), testosterone-3-(O-carboxymethyl)oxime (T-3-CMO) and testosterone-17β-hemisuccinate (T-17-HS) were tested for their usefulness as radiotracers in testosterone immunoassay. In the use of anti-15α- and 15β-CMT antisera produced in rabbits against 15α- and 15β-CMT-bovine serum albumin (BSA) conjugates, the antisera with 15α- and 15β-CMT-[^<125>I]iodinated tracers showed low sensitivity and somewhat low specificity in comparison with those of the antisera with tritiated testosterone (T-^3H). On the other hand, the antisear with T-3-CMO-[^<125>I]iodinated tracer showed high sensitivity but low specificity for 5α-dihydrotestosterone(5α-DHT)in comparison with T-^3H. The T-17-HS-[^<125>I]iodinated tracer was not bound to the antisera. In the use of anti-15α- and 15β-CMT antisera produced in rabbits by pretreatment with 15α-carboxymethyl-5α-DHT linked to a copolymer of D-glutamic acid and D-lysine followed by immunization with 15α- and 15β-CMT-BSA, the antisera with homologous[^<125>I]iodinated tracer showed high sensitivity and specificity.
- 公益社団法人日本薬学会の論文
- 1990-04-25
著者
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小嶋 正治
Faculty Of Pharmaceutical Sciences Kyusyu University
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三宅 義徳
Faculty Of Pharmaceutical Sciences Kyushu University
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重年 稔
Faculty of Pharmaceutical Sciences, Kyushu University
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重年 稔
Faculty Of Pharmaceutical Sciences Kyushu University
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小嶋 正治
Faculty Of Pharmaceutical Sciences Kyushu University
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