Is Recombinant Human Granulocyte Colony-Stimulating Factor (G-CSF) Orally Available in Rats?

概要

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Oral availability of recombinant human granulocyte colony-stimulating factor (G-CSF) was investigated in rats by measuring the blood total leucocyte (BTL) counts. Oral test G-CSF solution was prepared with 10% HCO-60^[○!R] (polyoxyethylated, 60μmol, castor oil derivative), 1% DK ester (sugar ester) or 10% MYS-40^[○!R] (polyethyleneglycol monostearate), in which the G-CSF concentration was 500 or 250μg/ml. Each test solution was injected into the duodenum of three rats at the G-CSF dose level of 300 or 600μg/kg, and BTL counts were monitored for 48h. All of the test G-CSF solution raised the BTL levels within 24h after injection. In particular, the HCO-60 solution increased the BTL levels over 2 times as compared to the predose level at 600μg/kg dose and the effect was apparently dose-dependent. A short-term study suggested that the effect of G-CSF on the BTL level appeared at the fastest at about 5h after administration of HCO-60 test solution, 300μg/kg. In view of the pattern of BTL dynamics obtained after i.v. injection of HCO-60 solution at 25 and 50μg/kg, the increase of BTL levels observed after oral administration of the HCO-60 solution is considered to be due to the orally supplied G-CSF.
公益社団法人日本薬学会の論文
1989-03-25